{"product_id":"abcam-ab267152","title":"Abcam, ab267152, Human MAP3K2 (MEKK2) knockout A549 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nMAP3K2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 7 and 2 bp deletion in exon 7. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 7 and 2 bp deletion in exon 7,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MAP3K2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMEKK2 also known as MAP3K2 is a kinase enzyme with an approximate mass of 70 kDa. It functions as part of the MAP kinase signaling pathway where it phosphorylates downstream proteins playing an essential role in transmitting signals within cells. This protein is mainly expressed in tissues such as the brain heart and liver. It acts by activating specific MAP kinase pathways facilitating various cellular responses to external stimuli.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis kinase serves as a critical mediator in cellular processes like proliferation differentiation and apoptosis. MEKK2 forms part of larger signaling complexes including the MAP3K complex where it regulates multiple cellular activities. Through its kinase activity MEKK2 passes on signals that affect gene expression and cellular stress responses impacting how cells react to their environment.\u003cbr\u003e\nPathways\u003cbr\u003e\nMEKK2 functions prominently in the MAPK signaling and NF-kB pathways. These pathways involve signal transduction mechanisms that MEKK2 influences through its interactions with related proteins such as MEK5 and ERK5. By modulating these pathways MEKK2 participates in a variety of cellular responses including inflammation and immune responses.\u003cbr\u003e\nMEKK2 has associations with conditions like cancer and inflammatory diseases. Aberrant expression of MEKK2 can affect the behavior of cancer cells due to its influence on the MAPK pathway's signaling leading to unchecked cell growth. Additionally MEKK2 interacts with proteins like NF-kB which are important in inflammation thereby implicating it in disorders related to immune system dysfunctions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845081747625,"sku":"ab267152","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab267152","provider":"Iright","version":"1.0","type":"link"}