{"product_id":"abcam-ab267293","title":"Abcam, ab267293, Human SPHK2 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSPHK2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SPHK2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSPHK2 also known as sphingosine kinase 2 is an enzyme responsible for the conversion of sphingosine to sphingosine-1-phosphate (S1P). This enzyme weighs approximately 68 kDa and is expressed in a wide range of tissues including liver kidney and brain. The catalytic activity of SPHK2 involves the phosphorylation of sphingosine which plays a part in the modulation of various cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSPHK2 influences cell growth survival and differentiation by generating sphingosine-1-phosphate a signaling lipid. It functions independently or in association with other proteins but it is not always a part of a larger complex. The generated S1P acts as a signaling molecule both inside and outside of cells affecting processes like apoptosis and inflammation.\u003cbr\u003e\nPathways\u003cbr\u003e\nSPHK2 is a significant player in the sphingolipid signaling pathway and impacts the MAPK\/ERK pathway. These pathways collectively regulate cellular proliferation and apoptosis. SPHK2 works in conjunction with SPHK1 a closely related sphingosine kinase in these signaling routes. These kinases together help to balance the levels of sphingosine and S1P which is essential for maintaining cellular homeostasis.\u003cbr\u003e\nSPHK2 has been linked to cancer and inflammatory diseases. The overexpression of SPHK2 can result in increased levels of sphingosine-1-phosphate promoting cancer cell survival and resistance to chemotherapy. In addition SPHK2's involvement in inflammation suggests roles in conditions like rheumatoid arthritis where it might interact with TNF-α related signaling pathways. These connections highlight SPHK2 as a potential target for therapeutic intervention in such diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845106847913,"sku":"ab267293","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab267293","provider":"Iright","version":"1.0","type":"link"}