{"product_id":"abcam-ab267619","title":"Abcam, ab267619, Human SIGLEC6 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman SIGLEC6 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SIGLEC6 in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.42 ng\/mL,\u003cbr\u003e\nRange:0.41 - 100 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman SIGLEC6 ELISA Kit is designed for the quantitative determination of SIGLEC6 in cell culture supernatants, plasma and serum samples.\u003cbr\u003e\nΔ Note:\u003cbr\u003e\nHuman SIGLEC6 concentration is low in normal serum\/plasma and may not be detectable in this assay.\u003cbr\u003e\nThis assay employs an antibody specific for human SIGLEC6 coated on a 96-well plate. Standards and samples are pipetted into the wells and SIGLEC6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human SIGLEC6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nThis ELISA kit shows no cross-reactivity with the following cytokines tested: Human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61\/CCN1, DAPP1, Dynactin Subunit 1\/DCTN1, DFF45\/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase1\/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2\/CD112, Nesfatin1\/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Pancreatic Polypeptide\/PP, Peroxiredoxin 2, PSMA1, PU.1\/Spi-1, RalA, RCOR1\/CoREST, Serpin B8, SH2D1A, SHP-1, SorCS3, THAP11, ULBP4\/RAET1E, UNC5H3, VAMP-1, VAMP-2, PBEF\/Visfatin.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSIGLEC6 also known as CD327 is a protein found on some immune cells particularly on B cells and to a lesser extent on macrophages and dendritic cells. This protein has a molecular mass of around 100 kDa. As a member of the sialic acid-binding immunoglobulin-type lectins family (siglecs) SIGLEC6 mechanistically functions by recognizing and binding to specific sialic acid-containing carbohydrates on cell surfaces. This recognition influences cell-cell interactions and signaling processes important for immune response modulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSIGLEC6 serves as an inhibitory receptor that helps dampen B cell receptor signaling balancing the immune response to prevent overactivation. It often partners with other proteins in complex cellular interactions within the immune cells. The expression of SIGLEC6 in placental tissues also suggests a role in trophoblast invasion and development although this function requires more research to fully understand the mechanisms involved.\u003cbr\u003e\nPathways\u003cbr\u003e\nSIGLEC6 is a part of the B cell receptor signaling pathway acting as an inhibitory modulator. Through this pathway it connects with proteins like CD22 and other siglecs that contribute to the regulation of B cell activity. Additionally its role in glycan recognition links it to pathways associated with cell surface interactions and immune tolerance.\u003cbr\u003e\nSIGLEC6 expression alterations have implications in chronic lymphocytic leukemia (CLL) where it potentially influences disease progression by modulating B cell signaling. The protein has also been connected to gestational trophoblastic diseases potentially affecting placental development. In these contexts SIGLEC6 interacts with proteins such as CD22 in CLL where they collectively affect leukemic cell behaviors and disease outcomes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843766046889,"sku":"ab267619","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab267619","provider":"Iright","version":"1.0","type":"link"}