{"product_id":"abcam-ab267630","title":"Abcam, ab267630, Mouse Calprotectin ELISA kit (S100A8\/S100A9)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse Calprotectin ELISA kit (S100A8\/S100A9) is a Sandwich (quantitative) ELISA for the measurement of Mouse Calprotectin (S100A8\/S100A9) in Mouse in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.65 ng\/mL,\u003cbr\u003e\nRange:0.614 - 150 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Calprotectin ELISA Kit is designed for the quantitative determination of Calprotectin in cell culture supernatants, plasma and serum samples.\u003cbr\u003e\nThis assay employs an antibody specific for mouse Calprotectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Calprotectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Calprotectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Calprotectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nS100A8 and S100A9 often known together as calprotectin are proteins that form a heterodimer and function as a complex. Each protein has a mass of approximately 10.8 kDa for S100A8 and 13.2 kDa for S100A9. These proteins express mainly in neutrophils and monocytes acting as calcium-binding proteins. Their expression can modulate various cellular functions like migration and inflammation responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCalprotectin serves as an important player in the immune response. This complex possesses antimicrobial properties and contributes to the body's defense by modulating inflammation. It binds to zinc and manganese to deprive pathogens of essential nutrients an action called nutrient bioavailability restriction. In immune cells calprotectin's presence signals the activation and recruitment of further immune components.\u003cbr\u003e\nPathways\u003cbr\u003e\nS100A8 and S100A9 influence the NF-kB signaling pathway which governs immune responses and inflammation. Their function affects other pathways such as MAPK by mediating pro-inflammatory cytokines' production and release. These proteins often engage with Toll-like receptors impacting the innate immune system and contributing to the body's defensive mechanisms against pathogens.\u003cbr\u003e\nHigh levels of calprotectin are associated with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. Its involvement in these conditions makes it a valuable biomarker. Calprotectin often interacts with other inflammatory molecules like TNF-alpha an important factor in propagating inflammation. The calprotectin ELISA test kit plasma calprotectin immunoassay and similar tools can aid in diagnosing and monitoring these conditions providing insight into disease progression and response to treatment.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843755102377,"sku":"ab267630","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab267630","provider":"Iright","version":"1.0","type":"link"}