{"product_id":"abcam-ab268978","title":"Abcam, ab268978, Recombinant human SPHK1 protein (Active)","description":"\u003cp\u003eSize: 5µg\u003cbr\u003e\nRecombinant human SPHK1 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with \u0026gt;70%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;70% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The specific activity of ab268978 was 500 nmol\/min\/mg in a kinase assay using sphingosine as substrate.,\u003cbr\u003e\nAccession:Q9NYA1,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSPHK1 also known as sphingosine kinase 1 is an enzyme that catalyzes the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P). This target has a molecular mass of approximately 47 kDa. SPHK1 is expressed in various tissues including the brain spleen heart and lungs. Its activity plays a role in cell signaling processes that are essential for cellular responses to external stimuli.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nWithin the cell SPHK1 participates in regulating cell survival proliferation and inflammation. It is part of the sphingolipid metabolic pathway which is not a traditional protein complex but more of a metabolic sequence. SPHK1 converts sphingosine a lipid signaling molecule into S1P which acts as a ligand for G-protein-coupled receptors. Through this conversion SPHK1 influences diverse cellular functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis protein actively participates in the sphingolipid signaling pathway and the apoptosis pathway. In sphingolipid signaling S1P acts alongside ceramide and other sphingolipids to modulate cell response. SPHK1's interplay with the ceramide-sphingosine-S1P rheostat can determine cell fate by tipping the balance towards survival or apoptosis. In the apoptosis pathway SPHK1 serves as a regulator of cell death with its function connected to proteins such as Bcl-2 and Bax which are primary apoptosis regulators.\u003cbr\u003e\nAbnormal expression of SPHK1 has links to cancer and cardiovascular diseases. In cancer overexpression of SPHK1 contributes to enhanced survival and proliferation of tumor cells with a noted interaction with the protein EGFR which is often upregulated in tumors. In cardiovascular diseases dysregulated SPHK1-S1P signaling can impact vascular integrity and inflammation influencing the progression of atherosclerosis with involvement of other inflammatory proteins like NF-kB.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843983495337,"sku":"ab268978","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab268978","provider":"Iright","version":"1.0","type":"link"}