{"product_id":"abcam-ab269823","title":"Abcam, ab269823, Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®","description":"\u003cp\u003eSize: 100µg \/ 3 x 10µg \/ 3 x 100µg \/ 1mg\u003cbr\u003e\nAlexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823) offers several standout features: - Rapid Conjugation: achieve Alexa Fluor® 647 labeling in under 20 minutes with just 30 seconds of hands-on time. - High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies. - Versatility: suitable for conjugating antibodies, proteins, and peptides. Alexa Fluor® 647 labeled antibodies can be used immediately in applications such as Western blot (WB), ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence: cited in over 40 publications.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nAlexa Fluor® 647 Conjugation Kit \/ Alexa Fluor® 647 Labeling Kit (ab269823) uses a simple and quick process for Alexa Fluor® 647 labeling \/ conjugation of antibodies. It can also be used to conjugate other proteins or peptides.\u003cbr\u003e\nTo conjugate an antibody to Alexa Fluor® 647 using this kit:\u003cbr\u003e\n- add modifier to antibody and incubate for 15 mins\u003cbr\u003e\n- add quencher and incubate for 5 mins\u003cbr\u003e\nThe Alexa Fluor® 647 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link\u003cbr\u003e\nRapid Alexa Fluor\u003cbr\u003e\n647 Labeling Kit. 337-0005 is the same as the 100 μg size. 336-0010 is the same as the 3 x 100 ug size. 336-0030 is the same as the 3 x 10 ug size. 336-0005 is the same as the 100 μg size. 336-0015 is the same as the 1 mg size.\u003cbr\u003e\nAmount and volume of antibody for conjugation to Alexa Fluor\u003cbr\u003e\nKit size\u003cbr\u003e\nRecommended\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum antibody\u003cbr\u003e\nvolume\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 20 μg\u003cbr\u003e\n3 x 10 μL\u003cbr\u003e\n100 μg\u003cbr\u003e\n100 μg\u003cbr\u003e\n200 μg\u003cbr\u003e\n100 μL\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 200 μg\u003cbr\u003e\n3 x 100 μL\u003cbr\u003e\n1 x 1 mg\u003cbr\u003e\n1 x 2 mg\u003cbr\u003e\n1 x 1 mL\u003cbr\u003e\nUsing the maximum amount of antibody may result in less labelling per antibody.\u003cbr\u003e\nIdeal antibody concentration is 1mg\/ml. 0.5 - 1 mg\/ml can be used if the maximum antibody volume is not exceeded. Antibodies \u0026gt; 2mg\/ml or \u0026lt; 0.5 mg\/ml should be diluted \/concentrated.\u003cbr\u003e\nBuffer Requirements for Conjugation\u003cbr\u003e\nBuffer should be pH 6.5-8.5.\u003cbr\u003e\nCompatible buffer constituents\u003cbr\u003e\nIf a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.\u003cbr\u003e\n50mM \/ 0.6% Tris\u003cbr\u003e\n0.1% BSA\u003cbr\u003e\n50% glycerol\u003cbr\u003e\n0.1% sodium azide\u003cbr\u003e\nPotassium phosphate\u003cbr\u003e\nSodium chloride\u003cbr\u003e\nSucrose\u003cbr\u003e\nSodium citrate\u003cbr\u003e\nTrehalose\u003cbr\u003e\nTris buffered saline is almost always ≤ 50 mM \/ 0.6%\u003cbr\u003e\nBSA can also interfere with the use of the conjugated antibody in tissue staining.\u003cbr\u003e\nIncompatible buffer constituents\u003cbr\u003e\nThiomerosal\u003cbr\u003e\nProclin\u003cbr\u003e\nGlycine\u003cbr\u003e\nArginine\u003cbr\u003e\nGlutathione\u003cbr\u003e\nIf a constituent of the buffer containing your antibody or protein is not listed above, please contact us.\u003cbr\u003e\nOnly purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.\u003cbr\u003e\nStoring and handling conjugation kits\u003cbr\u003e\nLyophilized Lightning-Link\u003cbr\u003e\ncomponents are hygroscopic.\u003cbr\u003e\nKits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.\u003cbr\u003e\nAlexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847592169641,"sku":"ab269823","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab269823","provider":"Iright","version":"1.0","type":"link"}