{"product_id":"abcam-ab270237","title":"Abcam, ab270237, Glutathione Affinity Resin - Amintra","description":"\u003cp\u003eSize: 10mL \/ 50mL \/ 100mL\u003cbr\u003e\nGlutathione Affinity Resin - Amintra® (ab270237) is designed for simple, rapid purification of Glutathione-S-transferase (GST)-tagged recombinant proteins produced using the pGEX series of expression vectors, other glutathione -S-transferases and glutathione binding proteins from bacteria, yeast, insect and mammalian cultures.\u003cbr\u003e\nKey facts\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 6 - 8Constituents: Agarose beads, 20% Ethanol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGlutathione Affinity Resin - Amintra\u003cbr\u003e\n(ab270237) is designed for simple, rapid purification of Glutathione-S-transferase (GST)-tagged recombinant proteins produced using the pGEX series of expression vectors, other glutathione -S-transferases and glutathione binding proteins from bacteria, yeast, insect and mammalian cultures. GST-tagged proteins can be purified directly from pre-treated bacterial lysates using Glutathione Affinity Resin - Amintra\u003cbr\u003e\n. The tagged proteins are eluted under mild, nondenaturing conditions that preserve protein antigenicity and function.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Amintra® Glutathione Affinity Resin. Expedeon product codes AGS0010 (10 mL Medium), AGS0025 (25 mL Medium) and AGS0100 (100 mL Medium) are the same as the current sizes: 10 mL, 25 mL, and 100 mL, respectively.\u003cbr\u003e\nThe glutathione ligand is coupled to highly cross-linked 4% agarose beads. The coupling is optimized to give high binding capacity for GST-tagged proteins and other glutathione binding proteins. Glutathione Affinity Resin - Amintra\u003cbr\u003e\ncan purify GST-tagged proteins under high flow rate. Its high flow properties make it excellent for scale-up.\u003cbr\u003e\nGlutathione Affinity Resin - Amintra\u003cbr\u003e\nis stable with all commonly used buffers and reagents including 0.1M NaOH and organic solvents. Removal of the GST tag can be performed whilst the fusion protein is bound to the column or in solution after elution.\u003cbr\u003e\nCharacterization of the Resin\u003cbr\u003e\nSupporting matrix:\u003cbr\u003e\n4% highly crosslinked agarose resin\u003cbr\u003e\nBead size range:\u003cbr\u003e\n45-165 μm\u003cbr\u003e\nRecommended working pH:\u003cbr\u003e\npH 3.0-12.0\u003cbr\u003e\nTypical binding capacity:\u003cbr\u003e\n\u0026gt; 10mg GST-tagged protein\/ml resin\u003cbr\u003e\nRecommended Flow rate*:\u003cbr\u003e\n100 – 300 cm\/h\u003cbr\u003e\nMaximum Flow rate:\u003cbr\u003e\n450 cm\/h\u003cbr\u003e\nMaximum pressure:\u003cbr\u003e\n3 bar, 43 psi\u003cbr\u003e\nChemical stability:\u003cbr\u003e\nSolubility in water:\u003cbr\u003e\nInsoluble\u003cbr\u003e\nLigand:\u003cbr\u003e\nThe glutathione ligand is coupled via a 12-atom linker\u003cbr\u003e\nLigand concentration:\u003cbr\u003e\n120-320 μmol glutathione\/ml medium\u003cbr\u003e\n* Binding of GST to glutathione is flow dependent and lower flow rates often increase the binding capacity. This is important during sample loading and elution. Protein characteristics, pH and temperature may also affect the binding\u003cbr\u003e\ncapacity.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847575163049,"sku":"ab270237","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab270237","provider":"Iright","version":"1.0","type":"link"}