{"product_id":"abcam-ab270601","title":"Abcam, ab270601, Protein A Gold Conjugate (20nm, 10 OD)","description":"\u003cp\u003eSize: 200µL \/ 1mL\u003cbr\u003e\nProtein A Gold Conjugate (20nm, 10 OD) (ab270601) is an aqueous suspension of 20 nm gold nanoparticles covalently bound with Protein A forming a highly stable conjugate. - Binds with high affinity to IgG subtypes - Proprietary surface coating shields the metallic core of the nanoparticle from the external environment, eliminating colloidal instability - Consistent high quality and batch-to-batch reproducibility - Available in different formats to fit your experimental needs\u003cbr\u003e\nKey facts\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nConjugation:20nm Gold,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nStorage buffer:pH: 7Constituents: Tris buffered saline, 0.1% Sorbitan monolaurate, ethoxylated\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProtein A Gold Conjugate (20nm, 10 OD) (ab270601) is manufactured using our Gold Nanoparticles which have a protective coating that can withstand the most extreme conditions (e.g. 2.5 M NaOH at 70°C for \u0026gt;1 hour). The irreversible covalent attachment does not allow the desorption of the Protein A, which is a major problem associated with gold conjugates when developed using passive adsorption techniques.\u003cbr\u003e\nTo exchange the conjugate into a specific buffer for your assay or test, centrifuge the conjugate in a microfuge at 9,000 x\u003cbr\u003e\nfor 20 minutes. Carefully remove the supernatant and add your preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).\u003cbr\u003e\nThe maximum absorbance for Protein A Gold Conjugate (20nm, 10 OD) is at 528 nm. To determine the effective concentration of the conjugate obtained we advise to measure the Absmax of light using an UV-vis spectrophotometer after diluting your sample to an appropriate range for your piece of equipment (e.g. if a 40 nm gold conjugate is at 10 OD and is diluted 1:10 the Abs530nm for a 1 cm light path is expected to be around 1 AU).\u003cbr\u003e\nBefore using Protein A Gold Conjugate (20nm, 10 OD) to detect a specific antibody we recommend that you check the affinity of Protein A for that antibody and subtype (see table below). Rabbit and human IgGs, for example, are good candidates because they bind to Protein A with high affinity.\u003cbr\u003e\nSpecies\u003cbr\u003e\nBinding Strength\u003cbr\u003e\nRabbit\u003cbr\u003e\nLow\/Medium\u003cbr\u003e\nLow\/Medium\u003cbr\u003e\nProtein A Gold Conjugate (20nm, 10 OD) is tested as a detector tool in Lateral Flow Assay but can also be used in electron and light microscopy, immunoblotting, and dot-blot. As Protein A Gold Conjugate (20nm, 10 OD) affinity for antibodies can vary, a titration of the specific antibody used is recommended in order to obtain the optimal performance. We recommend performing the incubation of Protein A Gold Conjugate (20nm, 10 OD)) with the antibody in a buffer that has a pH close to 8, for at least 10-15 minutes. After this stage do not centrifuge the gold complex.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company. It was previously called 20nm InnovaCoat\u003cbr\u003e\nGOLD (10 OD) Protein A Conjugate. Expedeon product code 224-0200 is the same as the 200 μl size of this product and code 224-1000 is the same as the 1 mL size.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844002435241,"sku":"ab270601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab270601","provider":"Iright","version":"1.0","type":"link"}