{"product_id":"abcam-ab270785","title":"Abcam, ab270785, Caspase 3\/7 Staining Kit (Far Red)","description":"\u003cp\u003eSize: 50Test\u003cbr\u003e\nCaspases play important roles in apoptosis and inflammation.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nCaspases play important roles in apoptosis and inflammation. Caspase 3\/7 Staining Kit (Far Red) are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells. The fluorescent 660-DEVD-FMK caspase-3\/7 inhibitor reagent allows researchers to assess caspase activation.\u003cbr\u003e\nThe fluorescent reagent 660-DEVD-FMK enters each cell and irreversibly binds to activated Caspase 3\/7. Because the 660-DEVD-FMK reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound 660-DEVD-FMK reagent diffuses out of the cell and is washed away. The remaining far red fluorescent signal is a direct measure of the active caspase enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound 660-DEVD-FMK can be analyzed by fluorescence microscopy or flow cytometry. Cells labeled with the 660-DEVD-FMK reagent may be read immediately or preserved for 16 hours using the fixative. Unfixed samples may also be analyzed with Hoechst stain to detect changes in nuclear morphology.\u003cbr\u003e\nProduced using a non-baculovirus expression system.\u003cbr\u003e\nThe target for this kit is Caspase 3\/7.\u003cbr\u003e\nNOTE: The number of tests per kit depends on the application being tested. This kit provides 50 tests for flow and 25 for imaging.\u003cbr\u003e\nFLICA 660-DEVD-FMK caspase-3\/7 inhibitor reagent contains the preferred binding sequence, Asp-Glu-Val-Asp (DEVD) for executioner caspases -3 and -7. This preferred caspase-3 and -7 binding sequence, DEVD, is labeled at the amino terminus end with a far-red fluorescent 660 dye and linked at the carboxyl end to a fluoromethyl ketone (FMK) reactive entity. The resulting cell permeant, fluorescent molecule, 660-DEVD-FMK, optimally excites at 660 nm and emits between 685-690 nm. A conventional red HeNe laser with a 633 nm excitation provides excellent excitation efficiency, enabling cells labeled with 660-DEVD-FMK to be analyzed with most flow cytometers, as well as fluorescence microscopes equipped with electronic grey scale image capabilities.\u003cbr\u003e\nKits are available for other caspases:\u003cbr\u003e\nPoly Caspase Staining Kit (Far Red) (\u003cbr\u003e\nab270783\u003cbr\u003e\nCaspase 1 Staining Kit (Far Red) (ab27084)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855604175017,"sku":"ab270785","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab270785","provider":"Iright","version":"1.0","type":"link"}