{"product_id":"abcam-ab273271","title":"Abcam, ab273271, Glycogen Phosphorylase Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGlycogen Phosphorylase Assay Kit ab273271 is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Adherent cells, Suspension cells, Tissue,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGlycogen Phosphorylase Assay Kit (Colorimetric) (ab273271) is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm. The OD 450 nm signal is directly proportional to the glycogen phosphorylase activity. The Glycogen Phosphorylase Colorimetric Assay Kit is rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity. The kit can detect as low as 10 mU in a variety of sample types.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K179 Biovision Glycogen Phosphorylase Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlycogen Phosphorylase also known as 'Phosphorylase' is an enzyme that catalyzes the breakdown of glycogen into glucose-1-phosphate. This enzyme exists in different isoforms including muscle liver and brain glycogen phosphorylases. The molecular mass of glycogen phosphorylase is approximately 97 kDa. It typically expresses in tissues that store glycogen such as liver and muscle enabling these tissues to convert stored glycogen into glucose during times of energy demand.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein plays a role in glycogen metabolism by releasing glucose units through the glycogen phosphorylase reaction. It is not part of a complex but functions independently. Glycogen phosphorylase activity increases during fasting or physical exertion providing an accessible source of glucose. This regulation is important for maintaining blood glucose levels especially in liver tissue where glycogen phosphorylase function is essential for homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlycogen phosphorylase is integral to glycogenolysis a part of carbohydrate metabolism. It works alongside other enzymes such as glycogen synthase in balancing the breakdown and synthesis of glycogen. In hormonal control pathways glycogen phosphorylase activity is modulated by epinephrine and glucagon which promote its conversion to the active form. Through these pathways phosphorylases exhibit dynamic responses to varying energy needs.\u003cbr\u003e\nGlycogen phosphorylase is linked to McArdle's disease a glycogen storage disorder resulting from muscle phosphorylase deficiency. This condition causes muscle pain and weakness upon exertion. Another related disorder is Hers disease caused by liver glycogen phosphorylase deficiency resulting in hypoglycemia and hepatomegaly. Both conditions highlight the importance of the glycogen phosphorylase function for normal muscle and liver physiology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843637760169,"sku":"ab273271","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab273271","provider":"Iright","version":"1.0","type":"link"}