{"product_id":"abcam-ab273310","title":"Abcam, ab273310, Transketolase Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nTransketolase Activity Assay Kit (Fluorometric) (ab273310) is a simple, plate-based fluorometric assay for measuring Transketolase (EC 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Purified protein, Tissue Lysate, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nSensitivity:= 5 µU,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTransketolase Activity Assay Kit (Fluorometric) (ab273310) is a simple, plate-based fluorometric assay for measuring Transketolase (EC 2.2.1.1) TKT activity in biological samples. In this assay, TKT transfers a two-carbon group from a donor keto sugar to an acceptor aldose sugar. The product formed converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction in the presence of a developer and an enzyme mix.\u003cbr\u003e\nThe assay can detect as low as 5 uU of TKT activity in biological samples.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K2004 Biovision Transketolase Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTransketolase (TKT) is an important enzyme that plays an important mechanical role in the pentose phosphate pathway by catalyzing the transfer of ketol groups from ketose donors to aldose acceptors. This process forms part of the non-oxidative segment of the pathway converting ribose 5-phosphate into other sugars equipped for nucleotide synthesis and energy metabolism. TKT is also recognized under the name erythrocyte transketolase as it is found in high abundance in erythrocytes. The enzyme has a molecular mass of around 73 kDa and expresses broadly in human tissues especially within liver and fat cells where it supports carbohydrate metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTransketolase participates in critical cellular metabolic processes facilitating cellular growth and repair by supporting the synthesis of nucleotides and amino acids. It does not function alone but rather operates as part of a complex with other enzymes such as transaldolase within the pentose phosphate pathway. The direct activity of erythrocyte transketolase can be essential for maintaining redox balance and providing intermediates that impact cell proliferation and antioxidant defense mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nWith regard to pathways involved in carbohydrate metabolism TKT plays an important role in the pentose phosphate pathway and glycolysis. In the pentose phosphate pathway TKT collaborates with enzymes like glucose-6-phosphate dehydrogenase to provide ribose-5-phosphate for nucleic acid synthesis and NADPH for reductive biosynthesis and oxidative stress defense. Its relationship with transaldolase in this pathway showcases a tightly regulated enzymatic network that maintains cellular homeostasis.\u003cbr\u003e\nTKT is associated with metabolic dysfunctions such as diabetes and certain neurodegenerative diseases including Wernicke's encephalopathy. In diabetes changes in transketolase activity can disturb glucose metabolism and contribute to oxidative stress linking it subconsciously with enzymes like phospholipase C which play roles in lipid signaling and cellular responses. Additionally reduced erythrocyte transketolase activity connects with thiamine deficiency in Wernicke's encephalopathy further affecting brain functions and metabolic pathways relying on proper transketolase function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843652047017,"sku":"ab273310","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab273310","provider":"Iright","version":"1.0","type":"link"}