{"product_id":"abcam-ab273339","title":"Abcam, ab273339, Glucosylceramidase Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGlucosylceramidase Activity Assay Kit (Fluorometric) ab273339 is a quantitative assay for the determination of Glucosylceramidase activity in tissue homogenates and cell lysates. Readout on any fluorometric (Ex\/Em=360\/445 nm) plate reader. - Kit includes standard curve for quantitation, and positive control enzyme. -Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Tissue Homogenate, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Glucosylceramidase Activity Assay Kit (Fluorometric) is a sensitive assay to measure the the activity level of the glucosylceramidase enzyme in a sample by quantifying the fluorescence intensity of a cleaved synthetic substrate in tissue homogenates \u0026amp; cell lysates.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nGlucosylceramidase Activity Assay Kit (Fluorometric) uses a synthetic substrate containing a fluorescent molecule, where the glucosylceramidase enzyme cleaves the substrate, releasing the fluorescent molecule which can then be quantified by measuring its fluorescence intensity (Ex\/Em=360\/445 nm), thus allowing for the determination of the enzyme's activity level in a sample; essentially, the more fluorescent signal detected, the higher the glucosylceramidase activity present.\u003cbr\u003e\nAssay protocol summary\u003cbr\u003e\nGlucosylceramidase Activity Assay Kit (Fluorometric) protocol summary\u003cbr\u003e\n- Prepare all reagents, samples and positive controls.\u003cbr\u003e\n- Add all samples to the appropriate wells.\u003cbr\u003e\n- Prepare and add Glucosylceramidase Substrate to the appropriate wells.\u003cbr\u003e\n- Measure fluorescence y (Ex\/Em = 360\/445 nm) at 37°C  in end point mode\u003cbr\u003e\n- Determine Glucosylceramidase activity using equation.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K2003 Glucosylceramidase Activity Assay Kit (Fluorometric). K2003-100 is the same size as the 100 test size of ab273339.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGBA also known as glucosylceramidase is a lysosomal enzyme with a molecular mass of approximately 59 kDa. This enzyme breaks down glucosylceramide into glucose and ceramide. GBA is expressed predominantly in tissues with high metabolic activities such as the brain liver and spleen. Its function relies on its catalytic activity where substrates bind to its active site enabling the hydrolysis process necessary for maintaining cellular metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGBA plays an important role in sphingolipid metabolism. It participates in the degradation of glycolipids within the lysosome contributing to lipid recycling. It acts independently rather than as a part of a major enzymatic complex. Through its role in degrading glucosylceramide GBA influences cellular homeostasis and bioenergetics ensuring balance in neural and systemic lipid levels.\u003cbr\u003e\nPathways\u003cbr\u003e\nGBA’s enzymatic functions are integral to the glycosphingolipid metabolic pathway. It is involved in the downstream steps of the lysosomal degradation of glycosphingolipids. The pathway operates alongside other important proteins such as beta-glucosidase and CERT-related transfer proteins all of which contribute to membrane lipid organization and signal transduction processes.\u003cbr\u003e\nGBA mutations are linked with Gaucher disease and Parkinson’s disease. In Gaucher disease deficient GBA activity leads to substrate accumulation resulting in hepatosplenomegaly and other systemic symptoms. Reduced GBA activity is also associated with increased alpha-synuclein aggregation in Parkinson’s disease implicating it in the pathogenesis of neurodegenerative disorders. The enzyme’s function in these diseases highlights its role in maintaining cellular equilibrium and signaling pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843631337641,"sku":"ab273339","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab273339","provider":"Iright","version":"1.0","type":"link"}