{"product_id":"abcam-ab273735","title":"Abcam, ab273735, Human CYP24A1 knockout A549 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nCYP24A1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR\/Cas9 X = 1 bp deletion Frameshift: 98%. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by CRISPR\/Cas9 X = 1 bp deletion Frameshift: 98%,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CYP24A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCYP24A1 also known as cytochrome P450 family 24 subfamily A member 1 is an enzyme with a molecular mass of approximately 56 kDa. It plays an important role in the catabolism of active vitamin D metabolites particularly in the hydroxylation of calcitriol. This enzyme is located mainly in the kidney but also in other tissues such as the placenta and the pituitary gland. Its expression is regulated by various physiological factors including calcium and phosphate levels.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis enzyme modulates the levels of active vitamin D influencing calcium and phosphate homeostasis. By converting calcitriol into its inactive forms CYP24A1 prevents excessive vitamin D activity which affects bone mineralization and cellular processes. It does not function as part of a larger complex but interacts closely with other vitamin D-metabolizing enzymes.\u003cbr\u003e\nPathways\u003cbr\u003e\nCYP24A1 is an integral component of the vitamin D metabolism pathway. It acts in concert with another enzyme CYP27B1 which activates vitamin D by hydroxylating calcidiol to calcitriol. Together they balance the hormone levels vital for various cellular functions. The enzyme also participates in calcium signaling pathways affecting cellular growth and differentiation.\u003cbr\u003e\nDysregulation of CYP24A1 has been associated with conditions such as hypercalcemia and certain cancers like colorectal cancer. Overexpression or mutations in this enzyme lead to altered vitamin D metabolism exacerbating disease states. In cancer CYP24A1 often interplays with proteins like VDR the vitamin D receptor influencing cancer cell proliferation and response to therapy.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845124870313,"sku":"ab273735","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab273735","provider":"Iright","version":"1.0","type":"link"}