{"product_id":"abcam-ab275045","title":"Abcam, ab275045, Apoptosis pathway knockout cell lysates panel","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nApoptosis pathway knockout cell lysates panel suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nA collection of 12 knockout cell lysates of protein targets involved in the apoptotic cell death pathways (mitochondrial and death receptor), available together for your convenience, with matching parental cell lysates.\u003cbr\u003e\nFind out more about our apoptosis assays\u003cbr\u003e\nIncluded in this panel:\u003cbr\u003e\nCASP8 knockout HeLa cell lysate (\u003cbr\u003e\nab256858\u003cbr\u003e\nFADD knockout HeLa cell lysate (\u003cbr\u003e\nab257261\u003cbr\u003e\nBAX knockout HeLa cell lysate (\u003cbr\u003e\nab263841\u003cbr\u003e\nPGAM5 knockout HeLa cell lysate (\u003cbr\u003e\nab257581\u003cbr\u003e\nTNFRSF10B knockout HeLa cell lysate (\u003cbr\u003e\nab257748\u003cbr\u003e\nHDAC6 knockout HeLa cell lysate (ab257145)\u003cbr\u003e\nRIPK2 (RIP2) knockout HeLa cell lysate (\u003cbr\u003e\nab258636\u003cbr\u003e\nFAS knockout HeLa cell lysate (\u003cbr\u003e\nab256911\u003cbr\u003e\nDAXX knockout HeLa cell lysate (\u003cbr\u003e\nab257408\u003cbr\u003e\nHSPB1 knockout HeLa cell lysate (\u003cbr\u003e\nab256945\u003cbr\u003e\nBRE knockout HeLa cell lysate (\u003cbr\u003e\nab257861\u003cbr\u003e\nMKI67 knockout HeLa cell lysate (\u003cbr\u003e\nab263762\u003cbr\u003e\nParts of the panels can be purchased separately. For further details please contact technical@abcam.com.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe target proteins Ki67 DR5 FADD Bax Daxx Fas RIP2 Caspase-8 Hsp27 HDAC6 BRCC45\/BRE and PGAM5 play various roles within cellular processes. Ki67 an antigen with a mass of about 395 kDa is a cellular marker for proliferation expressed in the cell nucleus during active phases of the cell cycle. DR5 (Death Receptor 5) part of the TNF receptor family is expressed on the cell membrane and engages in apoptosis pathways. FADD (Fas-Associated protein with Death Domain) acts as an adaptor molecule for death receptors. Bax a pro-apoptotic member of the Bcl-2 family is mainly located in the cytosol and mitochondria. Daxx (Death-domain associated protein) plays a role in apoptotic signaling and gene transcription modulation. Fas (CD95) often interacts with FADD to initiate apoptosis. RIP2 (Receptor-interacting serine\/threonine-protein kinase 2) is involved in signaling pathways related to immune response. Caspase-8 executes apoptotic cell death and inflammation. Hsp27 (Heat-shock protein 27) assists in protein folding and apoptosis inhibition. HDAC6 a histone deacetylase participates in the modulation of protein acetylation. BRCC45\/BRE is part of a complex that repairs DNA breaks. PGAM5 (Phosphoglycerate mutase family member 5) is a mitochondrial protein involved in metabolic processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThese proteins engage in cell regulation and apoptosis. Ki67 tracks cell proliferation important for tumor growth studies. DR5 often within receptor complexes triggers extrinsic apoptosis through ligand binding. FADD and Caspase-8 components of the death-inducing signaling complex (DISC) execute cell death. Bax promotes intrinsic apoptosis by permeabilizing mitochondrial membranes. Daxx modulates apoptosis and transcriptional repression. Fas engages FADD and Caspase-8 in apoptotic signaling. RIP2 participates in immune response signaling activating NF-kB pathway. Hsp27 a chaperone protein manages stress responses and inhibits apoptosis. HDAC6 orchestrates deacetylation of tubulin affecting cell mobility and stress response. BRCC45\/BRE is mechanistically part of the BRCA1-A complex important for DNA repair. PGAM5 operates in dephosphorylation influencing apoptosis and antioxidant defense.\u003cbr\u003e\nPathways\u003cbr\u003e\nThese proteins integrate into apoptosis and immune response pathways. Ki67 does not partake directly in a classic pathway but indicates active cell division. DR5 Fas FADD and Caspase-8 participate in the extrinsic pathway of apoptosis vital for removing harmful cells. Bax integrates into the intrinsic apoptotic pathway a response to cellular stress. Daxx and RIP2 contribute to the JNK and NF-kB pathways respectively influencing apoptosis and inflammation. HDAC6 impacts the response to oxidative stress and cellular mobility pathways. BRCC45\/BRE within BRCA1-A complex affects DNA damage response. PGAM5 plays roles in cellular stress response pathways integrating with related proteins such as Keap1.\u003cbr\u003e\nThese proteins have associations with cancer and neurodegenerative diseases. Abnormally increased expression of Ki67 often indicates aggressive tumors relating it to tumor progression. Dysregulation of DR5 Bax Fas FADD and Caspase-8 contributes to various cancers due to defective apoptosis. Daxx through its role in transcription regulation is linked to neurodegenerative disorders. RIP2's involvement in immune responses connects it to inflammatory diseases. HDAC6 by impacting protein folding and degradation ties into neurodegenerative diseases where misfolded proteins accumulate. BRCC45\/BRE mutations affect DNA repair fidelity linking it to breast cancer. PGAM5 has implications in neurodegenerative diseases through its modulation of mitochondrial function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845112254633,"sku":"ab275045","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab275045","provider":"Iright","version":"1.0","type":"link"}