{"product_id":"abcam-ab275754","title":"Abcam, ab275754, pCambia1300 Plant Expression Vector","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nVector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system.\u003cbr\u003e\nKey facts\u003cbr\u003e\nTarget:pCambia1300See target data,\u003cbr\u003e\nForm:LyophilizedSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: 71% Tris, 17% EDTA\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nVector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system.\u003cbr\u003e\nThe pCambia1300 vector offers:\u003cbr\u003e\nHigh copy number in\u003cbr\u003e\nE.coli\u003cbr\u003e\nfor high DNA yields\u003cbr\u003e\npVS1 replicon for high stability in\u003cbr\u003e\nAgrobacterium\u003cbr\u003e\nSmall size\u003cbr\u003e\nRestriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest\u003cbr\u003e\nBacterial selection with kanamycin\u003cbr\u003e\nPlant selection with hygromycin B\u003cbr\u003e\nPlant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV\u003cbr\u003e\nS promoter and terminated by the CaMV\u003cbr\u003e\nS polyA signal.\u003cbr\u003e\nThis vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes.\u003cbr\u003e\nThe pUC18 polylinker within the\u003cbr\u003e\na fragment allows blue\/white screening of clones in\u003cbr\u003e\nE. coli\u003cbr\u003e\ncloning work.\u003cbr\u003e\nGenbank Sequence data\u003cbr\u003e\nConcentration of Antibiotics for selection.\u003cbr\u003e\nIn bacterial system:\u003cbr\u003e\nKanamycin resistance (\u003cbr\u003e\naphIII\u003cbr\u003e\nnptIII\u003cbr\u003e\n\/3'5''-aminoglycoside phosphotransferase typeIII from\u003cbr\u003e\nEnterococcus faecalis\u003cbr\u003e\npJH1) in bacteria: use 50 μg\/mL in\u003cbr\u003e\nE.coli\u003cbr\u003e\nA.tumefaciens\u003cbr\u003e\nIn plant system:\u003cbr\u003e\nResistance Gene\u003cbr\u003e\n(From a\u003cbr\u003e\nKlebsiella\u003cbr\u003e\nspecies, via\u003cbr\u003e\nE.coli\u003cbr\u003e\nTransformed plant selection antibiotic\u003cbr\u003e\nHygromycin B\u003cbr\u003e\nConcentration in\u003cbr\u003e\nArabidopsis\u003cbr\u003e\n50 μg\/mL in germination medium\u003cbr\u003e\nConcentration in Rice\u003cbr\u003e\n50 μg\/mL in 2N6 then in RGH6\u003cbr\u003e\nConcentration in Tobacco\u003cbr\u003e\n50 μg\/mL in RMOP then in MST\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847577981097,"sku":"ab275754","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab275754","provider":"Iright","version":"1.0","type":"link"}