{"product_id":"abcam-ab275758","title":"Abcam, ab275758, pCambia2300 Plant Expression Vector","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nVector contains genes for bacterial and plant selection with kanamycin and and no reporter gene to allow construction of user's own system.\u003cbr\u003e\nKey facts\u003cbr\u003e\nTarget:pCambia2300See target data,\u003cbr\u003e\nForm:LyophilizedSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: 71% Tris, 17% EDTA\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nVector contains genes for bacterial and plant selection with kanamycin and and no reporter gene to allow construction of user's own system.\u003cbr\u003e\nThe pCambia2300 vector offers:\u003cbr\u003e\nHigh copy number in\u003cbr\u003e\nE.coli\u003cbr\u003e\nfor high DNA yields\u003cbr\u003e\npVS1 replicon for high stability in\u003cbr\u003e\nAgrobacterium\u003cbr\u003e\nSmall size\u003cbr\u003e\nRestriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest\u003cbr\u003e\nBacterial selection with kanamycin\u003cbr\u003e\nPlant selection with kanamycin\u003cbr\u003e\nPlant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV\u003cbr\u003e\nS promoter and terminated by the CaMV\u003cbr\u003e\nS polyA signal.\u003cbr\u003e\nThis vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes.\u003cbr\u003e\nThe pUC18 polylinker within the\u003cbr\u003e\nfragment allows blue\/white screening of clones in\u003cbr\u003e\nE.coli\u003cbr\u003e\ncloning work.\u003cbr\u003e\nGenbank Sequence data\u003cbr\u003e\nConcentration of Antibiotics for selection.\u003cbr\u003e\nIn bacterial system:\u003cbr\u003e\nKanamycin resistance (\u003cbr\u003e\naphIII\u003cbr\u003e\nnptIII\u003cbr\u003e\n\/3'5''-aminoglycoside phosphotransferase typeIII from\u003cbr\u003e\nEnterococcus faecalis\u003cbr\u003e\npJH1) in bacteria: use 50 μg\/mL in\u003cbr\u003e\nE.coli\u003cbr\u003e\nA.tumefaciens\u003cbr\u003e\nIn plant system:\u003cbr\u003e\nResistance Gene\u003cbr\u003e\nnptII\/aph(3')II\u003cbr\u003e\nfrom Tn5\u003cbr\u003e\nTransformed plant selection antibiotic\u003cbr\u003e\nKanamycin\u003cbr\u003e\nConcentration in\u003cbr\u003e\nArabidopsis\u003cbr\u003e\n20 - 50 μg\/mL\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847580471465,"sku":"ab275758","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab275758","provider":"Iright","version":"1.0","type":"link"}