{"product_id":"abcam-ab276185","title":"Abcam, ab276185, COVID-19 S-Protein (S1RBD) Human IgA ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nCOVID-19 S-Protein (S1RBD) Human IgA ELISA Kit is a Quantitative ELISA for the measurement of COVID-19 S-Protein (S1RBD) Human IgA in Human in Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nRange:1.37 - 1000 U\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCOVID-19 S-Protein (S1RBD) Human IgA ELISA Kit (ab276185) is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nindirect ELISA for the quantitative measurement of human IgA antibody against SARS-CoV-2 S1 RBD protein in human serum.\u003cbr\u003e\nStandard 96-well plates (12 strips with 8 wells\/strip) are coated with the SARS-CoV-2 S1 RBD protein, which combines with the corresponding antibody present in a sample and Positive control, which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of COVID19 S1 RBD protein human IgA antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nThe Positive Controls are from an inactivated serum sample which contains SARS-COV-2 S1 RBD protein human IgA antibody. We do not know the exact amount of SARS-COV-2 S1 RBD protein human IgA antibody in the Positive Control sample. The Positive Control can be used as a calibration curve for interpretation purposes in different assays.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe COVID-19 Spike Protein often referred to as SARS-CoV-2 Spike Protein is an important structural protein of the virus with a molecular mass around 180 kDa. This protein facilitates viral entry into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor which is primarily expressed in the respiratory tract. It's a trimeric protein composed of S1 and S2 subunits. The S1 subunit contains the receptor binding domain while the S2 subunit mediates membrane fusion.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe spike protein plays an important role in viral infectivity and host range determination. The spike protein is part of the larger viral particle complex that includes other structural proteins like the nucleocapsid protein. Its interaction with host cell receptors triggers conformational changes allowing viral entry and subsequent replication within the host cells. This process is pivotal for the virus's lifecycle.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe spike protein is integral to the viral entry pathway a critical step in the viral lifecycle. It intersects with the renin-angiotensin system (RAS) pathway through its interaction with the ACE2 receptor. This interaction not only facilitates viral entry but can also dysregulate the RAS pathway. Additionally the spike protein's activity is related to other viral proteins that assist in replication and assembly such as the envelope protein.\u003cbr\u003e\nThe spike protein is directly linked to COVID-19 the disease caused by SARS-CoV-2 infection. Its interaction with the ACE2 receptor has been associated with severe respiratory illnesses and potentially other conditions like cardiovascular disorders due to its effect on the RAS pathway. The nucleocapsid protein is also important in the virus's pathogenesis working together with the spike protein to establish infection and disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855629406377,"sku":"ab276185","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab276185","provider":"Iright","version":"1.0","type":"link"}