{"product_id":"abcam-ab277286","title":"Abcam, ab277286, SARS-CoV-2 (COVID-19) IgA ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nSARS-CoV-2 (COVID-19) IgA ELISA Kit is an indirect ELISA for the qualitative detection of IgA class antibodies against SARS-CoV-2 (COVID-19) in human plasma and serum samples. - Colorimetric readout - 450 nm - Works on any standard plate reader - Easy results interpretation\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Heparin Plasma, Serum,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nSARS-CoV-2 (COVID-19) IgA ELISA Kit (ab277286) is intended for the qualitative determination of IgA class antibodies against SARS-CoV-2 in human serum or plasma (citrate, heparin) to support the diagnosis of COVID-19 disease and constitutes a supplement to direct pathogen detection. In addition, serology can be used to collect epidemiological information on the prevalence of SARS-CoV-2.\u003cbr\u003e\nMicrotiter plates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450\/620 nm is read using an ELISA Microtiter plate reader.\u003cbr\u003e\nAntibody Isotypes and State of Infection:\u003cbr\u003e\nSerology\u003cbr\u003e\nSignificance\u003cbr\u003e\nCharacteristic of the primary antibody response.\u003cbr\u003e\nHigh IgM titer: → suggests a current or very recent infection.\u003cbr\u003e\nFollows IgM production.\u003cbr\u003e\nCharacteristic of the secondary antibody response.\u003cbr\u003e\nMay persist for several years.\u003cbr\u003e\nHigh IgG titer with low IgM titer: → may indicate past infection.\u003cbr\u003e\nProduced in mucosal linings throughout the body (⇒ protective barrier).\u003cbr\u003e\nUsually produced early in the course of the infection.\u003cbr\u003e\nDiagnostic Specificity:\u003cbr\u003e\nThe diagnostic specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. SARS-CoV-2 infections emerged in December 2019 in Wuhan, China. The expected prevalence values for German and US blood donor panels from before December 2019 therefore amount to 0 %. The determined positive results correspond to a specificity of 98.26 % (95 %-confidence interval: 93.86 % - 99.79 %).\u003cbr\u003e\nSample panel\u003cbr\u003e\nNo. patients (n)\u003cbr\u003e\nPositive\u003cbr\u003e\nEquivocal\u003cbr\u003e\nNegative\u003cbr\u003e\nSpecificity\u003cbr\u003e\n95% CI\u003cbr\u003e\nBlood donors Germany\u003cbr\u003e\nBlood donors US\u003cbr\u003e\n95.92%\u003cbr\u003e\n98.26%\u003cbr\u003e\n93.86%-99.79%\u003cbr\u003e\nDiagnostic Sensitivity:\u003cbr\u003e\nThe diagnostic sensitivity is defined as the probability of the assay of scoring positive in the presence of the specific analyte. 42 samples from 25 patients tested positive for SARS-CoV-2 RNA by RT-PCR were tested.\u003cbr\u003e\nDays post symptom onset\u003cbr\u003e\nNo. samples (n)\u003cbr\u003e\nPositive\u003cbr\u003e\nEquivocal\u003cbr\u003e\nNegative\u003cbr\u003e\nSensitivity (Eqv excluded)\u003cbr\u003e\n44.44%\u003cbr\u003e\n44.44%\u003cbr\u003e\ngreater than 12\u003cbr\u003e\nInterferences:\u003cbr\u003e\nThree clinical samples exhibiting differing reactivities were tested for interference with each substance listed in the Table below: a positive, a negative, and an equivocal sample. All samples exhibited a change of signal less than 15 % when tested with each potential interferant.\u003cbr\u003e\nInterferent\u003cbr\u003e\nConc. tested\u003cbr\u003e\nAlbumin\u003cbr\u003e\n60 mg\/mL\u003cbr\u003e\nBilirubin conjugated\u003cbr\u003e\n0.4 mg\/mL\u003cbr\u003e\nBilirubin unconugated\u003cbr\u003e\n0.4 mg\/mL\u003cbr\u003e\nCholesterol\u003cbr\u003e\n4 mg\/mL\u003cbr\u003e\nHemoglobin\u003cbr\u003e\n10 mg\/mL\u003cbr\u003e\nTriglycerides\u003cbr\u003e\n15 mg\/mL\u003cbr\u003e\nCross Reactivity:\u003cbr\u003e\n105 samples with antibody activities to potentially cross reacting parameters (including antibodies to several respiratory pathogens) were tested to evaluate the cross reactivity of the assay.\u003cbr\u003e\nSamples +ve to antibodies to\u003cbr\u003e\nNo. of samples (n)\u003cbr\u003e\nPositive\u003cbr\u003e\nEquivocal\u003cbr\u003e\nNegative\u003cbr\u003e\nAdenovirus\u003cbr\u003e\nParainfluenzavirus\u003cbr\u003e\nCandida albicans\u003cbr\u003e\nBordetella pertussis\u003cbr\u003e\nInfluenzavirus A\u003cbr\u003e\nInfluenzavirus B\u003cbr\u003e\nEnterovirus\u003cbr\u003e\nRespiratory syncytial virus\u003cbr\u003e\nChlamydia pneumoniae\u003cbr\u003e\nLegionella pneumoniae\u003cbr\u003e\nMycoplasma pneumoniae\u003cbr\u003e\nHaemophilus influenzae\u003cbr\u003e\nOther Coronavirus\u003cbr\u003e\nCross reactions with antibodies to adenovirus and influenzavirus cannot be excluded. Cross reactivity with other human coronaviruses should be considered for result interpretation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.\u003cbr\u003e\nThe nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843793342633,"sku":"ab277286","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab277286","provider":"Iright","version":"1.0","type":"link"}