{"product_id":"abcam-ab277455","title":"Abcam, ab277455, Human Thyroid Peroxidase\/TPO ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Thyroid Peroxidase\/TPO ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Thyroid Peroxidase\/TPO in Human in Cell Culture Media, Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.3 ng\/mL,\u003cbr\u003e\nRange:0.41 - 100 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Thyroid Peroxidase\/TPO ELISA Kit (ab277455) is an\u003cbr\u003e\nin-vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Human Thyroid Peroxidase\/TPO in serum, plasma (Collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay), and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human Thyroid Peroxidase\/TPO coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Thyroid Peroxidase\/TPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Thyroid Peroxidase\/TPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Thyroid Peroxidase\/TPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThyroid Peroxidase (TPO) also known as thyroperoxidase is an enzyme with a molecular mass of around 105 kDa. It is expressed in the thyroid gland's follicular cells. TPO plays a mechanical role in the synthesis of thyroid hormones by catalyzing iodination and coupling reactions needed to produce triiodothyronine (T3) and thyroxine (T4). These hormones are essential for regulating metabolism growth and development. The activity of TPO is an important step in thyroid hormone production.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTPO participates in the thyroid's hormone biosynthesis pathway. It forms a part of the multi-enzyme complex that includes thyroglobulin and iodide. The enzyme facilitates the iodination of tyrosine residues on thyroglobulin and the coupling of iodotyrosines important for creating active thyroid hormones. TPO's enzymatic function is important for maintaining appropriate levels of circulating T3 and T4 impacting various physiological processes in the body.\u003cbr\u003e\nPathways\u003cbr\u003e\nTPO contributes to the thyroid hormone synthesis pathway which is a critical pathway for metabolic regulation. It works in close association with other proteins such as thyroglobulin and sodium\/iodide symporter (NIS) to ensure efficient thyroid hormone production. TPO's involvement in these pathways indicates its importance in the endocrine system's normal function and homeostasis.\u003cbr\u003e\nTPO is often associated with autoimmune thyroid diseases like Hashimoto's thyroiditis and Graves' disease. High TPO antibodies such as anti-TPO antibodies are commonly detected in these conditions indicating an autoimmune response against the thyroid. The presence of high anti-thyroid peroxidase antibodies can lead to the destruction of thyroid tissue often resulting in hypothyroidism. TPO's connection to these diseases highlights its role in autoimmune conditions affecting thyroid function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855643005097,"sku":"ab277455","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab277455","provider":"Iright","version":"1.0","type":"link"}