{"product_id":"abcam-ab277616","title":"Abcam, ab277616, Recombinant human coronavirus SARS-CoV-2 Nsp14 methyltransferase protein (Active)","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRecombinant human coronavirus SARS-CoV-2 Nsp14 methyltransferase protein (Active) is a SARS-CoV-2 Full Length protein, in the 5926 to 6452 aa range, expressed in Escherichia coli, with \u0026gt;80%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;80% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The specific activity of ab277616 was 14.1 nmol\/min\/mg as per the activity assay protocol.,\u003cbr\u003e\nAccession:P0DTD1,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:SARS-CoV-2,\u003cbr\u003e\nStorage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 70.8662% Water, 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 1.34% Sodium phosphate, 0.0038% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNsp14 or non-structural protein 14 is an essential enzyme of the SARS-CoV-2 virus. This protein has a molecular weight of approximately 59 kDa and is expressed in infected host cells. It plays key roles mechanically as an exoribonuclease and an N7-methyltransferase. Nsp14 exhibits proofreading activity that ensures viral RNA synthesis accuracy. This dual function boosts the fidelity of the viral genome replication contributing to the stability of the virus.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNsp14 interacts with other non-structural proteins forming part of a multi-protein complex essential to the replication machinery of the virus. Its exoribonuclease activity corrects mismatches in the RNA sequence allowing high-fidelity replication. This proofreading capability of Nsp14 sets it apart from many RNA viruses lacking such a mechanism granting SARS-CoV-2 increased resistance to mutations.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe actions of Nsp14 integrate closely with the viral replication and transcription pathways. Nsp14 functions in conjunction with Nsp10 a co-factor that enhances its exonuclease activity. Nsp14's involvement in both methylation and proofreading ensures the successful translation and stability of viral mRNA. This positioning within the replication mechanism links it tightly to pathways vital for viral propagation and infection efficiency.\u003cbr\u003e\nNsp14 is significantly involved in the progression of COVID-19 caused by SARS-CoV-2. Its central role in viral replication makes it a critical target for antiviral drug development. By working with the Nsp12 RNA-dependent RNA polymerase Nsp14 supports the virus's persistence and pathogenicity. The protein's interaction in these processes underlies its potential as a target for treatments aimed at curbing the spread and impact of SARS-CoV-2 infections.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843968946345,"sku":"ab277616","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab277616","provider":"Iright","version":"1.0","type":"link"}