{"product_id":"abcam-ab279308","title":"Abcam, ab279308, Anti-NeuN antibody [EPR12763] - Mouse IgG2a (Chimeric) - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nMouse Recombinant Monoclonal NeuN antibody. Carrier free. Suitable for IHC-P, ICC, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12763,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), IHC-P, IP, WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab279308 is the carrier free version of\u003cbr\u003e\nab279296\u003cbr\u003e\nThis mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (\u003cbr\u003e\nab177487\u003cbr\u003e\n). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.\u003cbr\u003e\nPathways\u003cbr\u003e\nNeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.\u003cbr\u003e\nDisruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855395606697,"sku":"ab279308","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab279308","provider":"Iright","version":"1.0","type":"link"}