{"product_id":"abcam-ab279831","title":"Abcam, ab279831, Phospho-Insulin Receptor (Y1189) and Total Insulin Receptor ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhospho-Insulin Receptor (Y1189) and Total Insulin Receptor ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-Insulin Receptor (Y1189) and Total Insulin Receptor in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhospho-Insulin Receptor (Y1189) and Total Insulin Receptor ELISA Kit (ab279831) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Insulin Receptor protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.\u003cbr\u003e\nThis Sandwich ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the measurement of human phospho-Insulin Receptor (Y1189) and total Insulin Receptor. An anti-pan Insulin Receptor antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Insulin Receptor present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho Insulin Receptor (Y1189) antibody is added to detect phosphorylated Insulin Receptor. In the remaining wells, biotinylated anti-pan-Insulin Receptor antibody is used to detect pan Insulin Receptor. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP- conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin Receptor (Y1189) or pan Insulin Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe insulin receptor (INSR) is a transmembrane glycoprotein that functions as a receptor for insulin. Known also as CD220 the receptor has a molecular weight of about 320 kDa. It is widely expressed in insulin-sensitive tissues such as liver muscle and adipose tissues. The insulin receptor consists of two α subunits and two β subunits forming a heterotetramer. These subunits are located outside and inside the cell membrane respectively. The α subunits bind insulin while the β subunits possess tyrosine kinase activity important for downstream signaling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nInsulin receptor plays a role in facilitating the effects of insulin on glucose and lipid metabolism. When insulin a protein binds to the insulin receptor it undergoes a conformational change that activates its intrinsic kinase activity. This activation further leads to tyrosine phosphorylation of intracellular targets resulting in modulation of cellular functions. Insulin receptor also participates in the formation of signaling complexes through interaction with substrates like insulin receptor substrate (IRS) proteins which are important for the transmission of the insulin signal inside cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nInsulin receptor is central to the insulin signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. Activation of the insulin receptor triggers the insulin signaling cascade which involves various proteins like PI3 kinase and Akt that contribute to glucose uptake and metabolism. In the MAPK pathway the insulin receptor influences gene expression related to cell growth and differentiation. These pathways intertwine with other hormone signaling systems and affect numerous physiological processes critical for maintaining metabolic homeostasis.\u003cbr\u003e\nAberrations in insulin receptor function are linked to insulin resistance and type 2 diabetes. Defective signaling through this receptor impairs glucose transport into cells contributing to hyperglycemia. Disorders in insulin receptor activity also relate to metabolic syndrome a cluster of conditions increasing the risk of heart disease. Proteins connected to these conditions include IRS proteins and PI3 kinase highlighting the integrated roles these components play in metabolic regulation. Developing anti-insulin receptor antibodies offers potential therapeutic interventions to modulate receptor function and manage these disorders effectively.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855656931497,"sku":"ab279831","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab279831","provider":"Iright","version":"1.0","type":"link"}