{"product_id":"abcam-ab280097","title":"Abcam, ab280097, Human CHI3L1 knockout THP-1 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nCHI3L1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 7.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:THP-1,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Blood,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 7,\u003cbr\u003e\nDisease:Acute Monocytic Leukemia\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CHI3L1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nYKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nYKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nYKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.\u003cbr\u003e\nYKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845096198313,"sku":"ab280097","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab280097","provider":"Iright","version":"1.0","type":"link"}