{"product_id":"abcam-ab280127","title":"Abcam, ab280127, Human ERCC4 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nERCC4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ERCC4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nXPF also known as ERCC4 is a protein that plays a mechanical role in DNA repair by cleaving DNA at junctions between single-stranded and double-stranded DNA. It exhibits a molecular mass of approximately 103 kDa. This protein is expressed in various tissues with high levels found in the liver and kidney. XPF functions as a DNA endonuclease significantly influencing genomic stability through its activity in DNA repair processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe XPF protein collaborates with ERCC1 to form a heterodimeric complex important for nucleotide excision repair (NER). This complex is essential in recognizing and repairing bulky DNA adducts therefore maintaining DNA integrity. The complex performs specific incisions near DNA damage sites removing lesions that frequently occur due to environmental factors like UV radiation and chemical pollutants.\u003cbr\u003e\nPathways\u003cbr\u003e\nXPF functions within the NER and interstrand crosslink repair pathways. In these pathways the XPF-ERCC1 complex coordinates with other proteins like XPA and RPA to accurately excise damaged DNA segments and facilitate repair synthesis. These interactions are important for restoring normal DNA configuration and function after damage thereby preventing mutations and genomic instability.\u003cbr\u003e\nMutations in XPF are linked to xeroderma pigmentosum group F (XP-F) and the Cockayne syndrome. XP-F is a disorder characterized by extreme sensitivity to sunlight and an increased risk of skin cancer due to impaired DNA repair capability. In Cockayne syndrome patients experience growth defects and neurological degeneration. Both of these disorders involve dysfunctional DNA repair mechanisms where proteins like ERCC1 and others in the repair pathways fail to adequately compensate for the defective XPF function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845085909161,"sku":"ab280127","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab280127","provider":"Iright","version":"1.0","type":"link"}