{"product_id":"abcam-ab281847","title":"Abcam, ab281847, Alexa Fluor® 488 Anti-Ki67 antibody [SP6]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-Ki67 antibody [SP6] - Alexa Fluor® 488 conjugated (ab281847) is a rabbit recombinant monoclonal antibody detecting Ki67 in  Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human, Mouse . - Clone SP6 is the most cited clone to Ki67 - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:SP6,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nConjugation:Alexa Fluor® 488,\u003cbr\u003e\nExcitation\/Emission:Ex: 495nm, Em: 519nm,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:ICC\/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAlexa Fluor® 488 Anti-Ki67 antibody [SP6] (ab281847) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse samples.\u003cbr\u003e\nCollaboration\u003cbr\u003e\nAnti-Ki67 [SP6] Alexa Fluor® 488 (ab281847) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nRelated products\u003cbr\u003e\nAntibody clone SP6 is also available pre-conjugated to a variety of labels for your convenience – Anti-Ki67 Alexa Fluor® 488 [SP6] (ab281847).\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [SP6] also available for your convenience:\u003cbr\u003e\nab16667\u003cbr\u003e\nab21700\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab197547\u003cbr\u003e\nab231172\u003cbr\u003e\n, ab238624, Oligonucleotide - ab279361, Alexa Fluor® 488 - ab281847, Alexa Fluor® 647 -\u003cbr\u003e\nab281928\u003cbr\u003e\n, PE -\u003cbr\u003e\nab282173\u003cbr\u003e\n, APC -\u003cbr\u003e\nab314285\u003cbr\u003e\nab320709\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab320710\u003cbr\u003e\n, ab323292\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nAlexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle, Store in the dark\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nKi67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.\u003cbr\u003e\nPathways\u003cbr\u003e\nKi67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K\/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.\u003cbr\u003e\nKi67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844157591721,"sku":"ab281847","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab281847","provider":"Iright","version":"1.0","type":"link"}