{"product_id":"abcam-ab282634","title":"Abcam, ab282634, Human SIRT5 knockout A549 cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial\u003cbr\u003e\nSIRT5 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,Western blot,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAlthough we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.\u003cbr\u003e\nRecommended control\u003cbr\u003e\n: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SIRT5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSIRT5 also known as Sirtuin 5 is a mitochondrial protein with enzymatic activity as a lysine desuccinylase demalonylase and deglutarylase. It has a molecular mass of approximately 34 kDa. This protein is expressed highly in tissues with high metabolic activity like the liver heart and brain where it participates in regulating energy metabolism. The activity of SIRT5 affects cellular processes by removing post-translational modifications from lysine residues modulating proteins' functions and interactions in these tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSIRT5 functions as an important regulator of mitochondrial metabolism and influences cellular energy homeostasis. It is not part of larger complexes but it interacts with various metabolic enzymes to control their function. Through its deacylase activity SIRT5 affects the urea cycle and fatty acid oxidation by modifying key enzymes involved in these processes. Its role in maintaining a balance in energy production and consumption highlights its importance in cellular metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nSIRT5 is involved in both the tricarboxylic acid (TCA) cycle and the regulation of reactive oxygen species (ROS). Within the TCA cycle SIRT5 desuccinylates and activates enzymes like citrate synthase enhancing the cycle's efficiency. In regulating ROS SIRT5 reduces oxidative stress by affecting enzymes such as superoxide dismutase 2 (SOD2). Its interaction with these pathways and proteins reflects its role in maintaining metabolic health and cellular integrity.\u003cbr\u003e\nAlterations in SIRT5 levels have been connected to metabolic diseases such as obesity and neurodegenerative disorders like Alzheimer's disease. In obesity SIRT5 dysregulation can lead to inefficient metabolic processes and energy imbalance. In Alzheimer’s decreased SIRT5 activity may contribute to increased mitochondrial oxidative stress implicated in disease progression. These connections highlight SIRT5’s significance in both metabolic and neurodegenerative contexts linking with proteins like AMPK in obesity and tau proteins in Alzheimer's disease.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845116383401,"sku":"ab282634","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab282634","provider":"Iright","version":"1.0","type":"link"}