{"product_id":"abcam-ab285238","title":"Abcam, ab285238, Dopamine ELISA Kit","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nDopamine ELISA kit is a competitive ELISA designed to quantify dopamine (DA) with a sensitivity of \u0026lt;0.938 ng\/mL.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids,\u003cbr\u003e\nAssay type:Competitive,\u003cbr\u003e\nSensitivity:\u0026lt; 0.938 ng\/mL,\u003cbr\u003e\nRange:1.56 - 100 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nDopamine ELISA kit ab285238 is a competitive ELISA designed to quantify dopamine (DA) with a sensitivity of \u0026lt;0.938 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nDopamine ELISA kit ab285238 is a competitive ELISA. There are 2 types of competitive ELISAs:\u003cbr\u003e\nantigen down\u003cbr\u003e\n: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.\u003cbr\u003e\nantibody down\u003cbr\u003e\n: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.\u003cbr\u003e\nDopamine ELISA kit ab285238 protocol summary\u003cbr\u003e\nAdd standards or samples to the wells, incubate 2 hrs\u003cbr\u003e\nWash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr\u003cbr\u003e\nWash, then add 50 µl of SP Conjugate per well. Incubate 30 min\u003cbr\u003e\nWash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min\u003cbr\u003e\nAdd 50 µl of Stop Solution per well. Read at 450 nm immediately\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843646148777,"sku":"ab285238","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab285238","provider":"Iright","version":"1.0","type":"link"}