{"product_id":"abcam-ab285263","title":"Abcam, ab285263, Mouse Alanine Aminotransferase (ALT) ELISA Kit","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nMouse Alanine Aminotransferase (ALT) ELISA Kit is a sandwich ELISA designed to quantify Mouse Alanine Aminotransferase (ALT) with a sensitivity of 0.938 mIU\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 1.56 - 100 mIU\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 0.938 mIU\/mL,\u003cbr\u003e\nRange:1.56 - 100 mIU\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Alanine Aminotransferase (ALT) ELISA Kit ab285263 is a sandwich ELISA to measure Mouse Alanine Aminotransferase (ALT) in serum, plasma, cell culture supernatant with a sensitivity of 0.938 0.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe density of color is proportional to the amount of mouse GPT captured from the samples.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse Alanine Aminotransferase (ALT) ELISA Kit ab285263 protocol summary\u003cbr\u003e\n1. Wash plate with wash solution\u003cbr\u003e\n2. Add standards and samples into appropriate wells. Incubate then discard\u003cbr\u003e\n3. Add Biotin detection antibody and incubate,\u003cbr\u003e\n4. Wash\u003cbr\u003e\nAdd SABC solution and incubate\u003cbr\u003e\n5. Wash\u003cbr\u003e\n6. Add TMB solution and incubate\u003cbr\u003e\n7. Add Stop solution and read at 450 nm\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called E4324 Alanine Aminotransferase 1 (GPT) (Mouse) ELISA Kit. E4324-100 is the same size as the 96 test size of ab285263.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.\u003cbr\u003e\nAlanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843621474473,"sku":"ab285263","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab285263","provider":"Iright","version":"1.0","type":"link"}