{"product_id":"abcam-ab285308","title":"Abcam, ab285308, Rat Myeloperoxidase ELISA Kit","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nRat Myeloperoxidase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Myeloperoxidase in Rat in Biofluids, Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Homogenate, Serum,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 0.469 ng\/mL,\u003cbr\u003e\nRange:0.78 - 50 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMyeloperoxidase (MPO) is a peroxidase enzyme most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. MPO contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H₂O₂) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). MPO also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.\u003cbr\u003e\nThis Rat Myeloperoxidase ELISA kit (ab285308, E4581) is a sandwich ELISA assay for the quantitative measurement of MPO in Rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of MPO captured from the samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMyeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.\u003cbr\u003e\nPathways\u003cbr\u003e\nMyeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.\u003cbr\u003e\nDysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855623311529,"sku":"ab285308","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab285308","provider":"Iright","version":"1.0","type":"link"}