{"product_id":"abcam-ab286655","title":"Abcam, ab286655, Human POLQ knockout A549 cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial \/ 2 x 1000000Cells \/ vial\u003cbr\u003e\nPOLQ KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-POLQ, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDNA Polymerase theta also known as pol θ and encoded by the POLQ gene is a specialized enzyme that plays a role in DNA repair processes. It has a molecular mass of approximately 290 kDa. This enzyme functions by adding nucleotides to a DNA strand and excising incorrect bases ensuring the maintenance of genomic integrity. Pol θ is largely expressed in cells with proliferative capacity such as those found in the testes and certain types of tumor tissues. The enzyme's ability to perform template-independent nucleotide addition is unique among polymerases.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDNA repair processes depend heavily on DNA Polymerase theta. This enzyme participates in a distinctive mechanism known as microhomology-mediated end-joining (MMEJ) which repairs double-strand breaks in DNA. Unlike canonical end-joining pathways MMEJ employs short homologous sequences to mediate repair where Pol θ serves a critical role. Although Pol θ acts independently it can still interact with other repair factors to ensure efficient DNA end-joining highlighting its importance.\u003cbr\u003e\nPathways\u003cbr\u003e\nMaintenance of genome stability relies on pathways involving DNA Polymerase theta. The enzyme is an important component of the MMEJ pathway an alternative mechanism to the more common non-homologous end-joining (NHEJ). In this capacity Pol θ cooperates with proteins involved in DNA damage response such as BRCA1 and BRCA2 which help facilitate repair processes and support cell survival under genotoxic stress conditions.\u003cbr\u003e\nDNA Polymerase theta has connections to cancer progression and resistance to DNA-damaging chemotherapy. Overexpression of Pol θ is common in various tumors such as breast and ovarian cancers where it promotes cell survival following DNA damage. The enzyme's interaction with the BRCA1 and BRCA2 proteins further implicates it in hereditary breast cancer syndromes suggesting a complex relationship with repair deficiencies and highlighting its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845194698921,"sku":"ab286655","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab286655","provider":"Iright","version":"1.0","type":"link"}