{"product_id":"abcam-ab286903","title":"Abcam, ab286903, Thrombin Sepharose Beads","description":"\u003cp\u003eSize: 5mL\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called 7925 Thrombin Sepharose Beads.\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: 50% Glycerol (glycerin, glycerine)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called 7925 Thrombin Sepharose Beads. 7925-1 is the same size as the 1 ml size of ab286903.\u003cbr\u003e\nBeads for convenient cleavage and purification of recombinant fusion proteins containing thrombin-specific cleavage sites.\u003cbr\u003e\nThrombin Sepharose beads are designed for efficient cleavage of recombinant fusion proteins, containing a thrombin cleavage site (e.g. Leu-Val-Pro-Arg-ll-Gly-Ser; ll: cleavage site), circumventing the need for chromatographic techniques to remove thrombin after cleavage reaction is completed. Thrombin Sepharose is prepared by covalent coupling of bovine thrombin to activated 6% cross-linked Sepharose beads. 15 µl of the slurry is sufficient to cleave \u0026gt;90% of 1 mg of 6xHis-tagged fusion protein in 50 mM Tris buffer, 0.1 M NaCl, pH 8.0 within 4 h at room temperature. These beads can be regenerated for repeat use. The reusability depends on multiple criteria including the usage and handling of the product\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847577096361,"sku":"ab286903","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab286903","provider":"Iright","version":"1.0","type":"link"}