{"product_id":"abcam-ab300722","title":"Abcam, ab300722, Calcineurin B\/PPP3R1 Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nCalcineurin B\/PPP3R1 Antibody Pair - BSA and Azide free is a matched antibody pair validated for use in sandwich-based immunoassays. - Contains a capture and a detector antibody at a concentration of approx. 1mg\/mL. - Carrier free (PBS only) - formulation: ideal for optimal conjugation to your label of choice - Antibody orientation based on internal optimization for ELISA-based assays - Capture and detector antibodies also available to purchase individually.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Rat, Mouse, Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nRange:0.16 - 20 ng\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThis Antibody Pair can be used to quantify Human Calcineurin B\/CNB. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg\/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.\u003cbr\u003e\nFor additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA\u003cbr\u003e\nab233621\u003cbr\u003e\n), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCalcineurin B also known as PPP3R1 serves as the regulatory subunit of the calcineurin phosphatase complex. The protein possesses a molecular mass of roughly 19 kDa. It expresses widely in various tissues especially in the brain skeletal muscle and immune cells. Calcineurin B partners with the catalytic subunit Calcineurin A to form the complete enzyme necessary for its dephosphorylation activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCalcineurin B plays an important role in regulating calcium signaling pathways. It functions as part of a heterodimeric complex where it binds calcium ions and is necessary for activating the serine\/threonine phosphatase activity of calcineurin. Through this activity calcineurin B influences the transcriptional activation of NFAT (Nuclear Factor of Activated T-cells) and other downstream signaling pathways affecting cell growth differentiation and immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nCalcineurin B acts critically in the calcium\/calmodulin-dependent signaling pathway and the T-cell receptor signaling pathway. In these pathways it regulates the activity of proteins like NFAT and influences downstream effects essential for immune responses. The dephosphorylation activity facilitated by calcineurin B also connects it with a network of signaling cascades modulating calcium levels impacting cellular activities significantly.\u003cbr\u003e\nCalcineurin B is associated with immunosuppressive outcomes and neurodegenerative conditions. Its connection with NFAT makes it a target for immune-related disorders such as autoimmune diseases and transplant rejection. Additionally alterations in its activity connect with proteins like tau contributing to disorders like Alzheimer's disease due to dysregulated calcium signaling and neuronal damage.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843598209193,"sku":"ab300722","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab300722","provider":"Iright","version":"1.0","type":"link"}