{"product_id":"abcam-ab304936","title":"Abcam, ab304936, Human Glutathione Peroxidase 4 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Glutathione Peroxidase 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Glutathione Peroxidase 4 with a sensitivity of 124 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 124 pg\/mL,\u003cbr\u003e\nRange:390.625 - 25000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Glutathione Peroxidase 4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glutathione Peroxidase 4 protein in human cell extracts. Quantitate Human Glutathione Peroxidase 4 with 124 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlutathione Peroxidase 4 (GPX4) also known as PHGPx acts as an important enzyme in protecting cells from oxidative damage by reducing lipid hydroperoxides to their alcohols and free hydrogen peroxide to water. It has a mass of approximately 20 kDa. GPX4 is widely expressed in various tissues including the brain testis kidney and liver underlining its significance in different physiological processes. GPX4 is essential in cellular anti-oxidant defense making it a potential marker in studies using GPX4 western blot techniques.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGPX4 plays an important role in protecting cellular membranes from lipid peroxidation by acting alone which differentiates it from other glutathione peroxidases that work in a complex. It is necessary for the production of glutathione products and helps maintain redox homeostasis by converting glutathione peroxides. This action prevents cell death pathways linked to oxidative stress and ensures cellular integrity particularly in sensitive tissues like the brain and reproductive organs.\u003cbr\u003e\nPathways\u003cbr\u003e\nGPX4 integrates into important cellular antioxidant pathways. Specifically it functions within the glutathione metabolic pathway collaborating with related proteins like glutathione synthetase and glutathione reductase. GPX4 is also involved in ferroptosis regulation a cell death pathway critically linked to glutathione levels. It helps balance redox-sensitive signaling and metabolic activities demonstrating synergy with other antioxidant enzymes in the cellular response to oxidative stress.\u003cbr\u003e\nResearch connects GPX4 deficiency to neurological disorders and cancer. Its important antioxidative activity makes it significant in preventing neurodegenerative diseases such as Alzheimer's where it interacts with proteins like PINK1. GPX4 also shows relevance in cancer as its altered expression may increase vulnerability to cell death suggesting its potential role in chemotherapy resistance and therapy development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843573895337,"sku":"ab304936","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab304936","provider":"Iright","version":"1.0","type":"link"}