{"product_id":"abcam-ab304943","title":"Abcam, ab304943, Human Cathepsin G ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Cathepsin G ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cathepsin G with a sensitivity of 101.065 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 101.065 pg\/mL,\u003cbr\u003e\nRange:468.75 - 30000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Cathepsin G SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin G protein in human cell extracts and cell lysates. Quantitate Human Cathepsin G with 101.065 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCathepsin G also known as CTSG is a serine protease with a molecular mass of approximately 29 kDa. It is primarily located in azurophilic granules of neutrophils a type of white blood cell. Cathepsin G functions mechanically as an enzyme cleaving peptide bonds in proteins and shaping the protein components involved in various immune responses. Its enzymatic activity is important for host defense processes including bacterial degradation and immune cell signaling. Researchers easily measure and study 'G activity' using specific functional assays.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCathepsin G plays a significant role in the immune system by contributing to inflammatory responses and regulating cellular processes through its proteolytic activity. It participates in activating other proteins such as anti-bacterial serine enzymes and breaking down extracellular matrix components during immune response facilitation. While not part of a larger complex cathepsin G works alongside other proteases showing combinatory effects in innate immunity.\u003cbr\u003e\nPathways\u003cbr\u003e\nCathepsin G is involved in the immune and inflammatory pathways. It plays a part in the regulation of inflammatory responses and contributes to the activation of cytokines during infection and tissue injury. Cathepsin G connects through shared activity with other proteases like neutrophil elastase influencing processes in tissue remodeling and cytokine release. It sustains immune system homeostasis maintaining a balance between inflammation and tissue repair in these pathways.\u003cbr\u003e\nCathepsin G is linked to chronic inflammatory conditions and autoimmune disorders. For example rheumatoid arthritis showcases the enzyme's connection where cathepsin G contributes to tissue damage and joint inflammation. Cathepsin G's increased activity associates with neutrophil elastase aggravating symptoms of chronic obstructive pulmonary disease (COPD) as it contributes to lung tissue degradation. Understanding cathepsin G's role in these diseases aids research in therapeutic target development focusing on 'E-64d' inhibitors. These inhibitors reduce inflammatory damage by curbing excessive protease activity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843580317865,"sku":"ab304943","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab304943","provider":"Iright","version":"1.0","type":"link"}