{"product_id":"abcam-ab307479","title":"Abcam, ab307479, Recombinant Human Factor D\/CFD Protein (His tag) (Active)","description":"\u003cp\u003eSize: 10µg \/ 50µg \/ 100µg\u003cbr\u003e\nRecombinant Human Factor D\/CFD Protein (His tag) (Active) is a Human Full Length protein, in the 26 to 253 aa range, expressed in HEK 293 cells, with \u0026gt;95%, \u0026lt;0.005 EU\/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% HPLC,\u003cbr\u003e\nEndotoxin level:\u0026lt;0.005 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:Mass Spec, HPLC, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Fully active determined by its ability to cleave colorimetric substrate N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl) in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is ≥ 149 pmol\/min\/ug.,\u003cbr\u003e\nAccession:P00746,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in PBS,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFactor D also known as complement factor D or CFD protein is a serine protease involved in the alternative pathway of the complement system. It plays a mechanical role by cleaving factor B when it is bound to C3b forming the active C3 convertase C3bBb. This enzyme is relatively small with a mass of approximately 28.5 kDa. It is mainly expressed in adipose tissue but it can also be found in the blood plasma.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFactor D aids the immune system's ability to destroy pathogens. As part of the alternative complement pathway it acts independently and does not associate with larger protein complexes. Its activation of the alternative pathway leads to direct lysis of pathogens therefore contributing to innate immunity.\u003cbr\u003e\nPathways\u003cbr\u003e\nFactor D participates specifically in the complement activation pathway particularly in the innate immune response. It works closely with other proteins in the complement system such as factor B and C3. This pathway enhances the opsonization and removal of microbes and damaged cells acting in parallel with the classical pathway of the complement system.\u003cbr\u003e\nAbnormal function or regulation of factor D can relate to chronic inflammation and age-related macular degeneration. Disorders involving factor D may involve excess complement activation leading to tissue damage. The role of factor D in these diseases often connects it to other key complement components such as C3 and factor B which can also become problematic when dysregulated.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843831550121,"sku":"ab307479","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab307479","provider":"Iright","version":"1.0","type":"link"}