{"product_id":"abcam-ab309444","title":"Abcam, ab309444, Human GLi3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman GLi3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GLi3 in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum, Plasma, Cell culture supernatant,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 40 pg\/mL,\u003cbr\u003e\nRange:40 - 10000 pg\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman GLi3 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GLi3 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GLi3 coated on a 96-well plate.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.\u003cbr\u003e\nPathways\u003cbr\u003e\nGli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.\u003cbr\u003e\nMutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843595587753,"sku":"ab309444","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab309444","provider":"Iright","version":"1.0","type":"link"}