{"product_id":"abcam-ab309595","title":"Abcam, ab309595, Anti-Calcium-independent Phospholipase A2\/PLA2G6 antibody [EPR26992-82]-BSA and Azide free(Capture)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Calcium-independent Phospholipase A2\/PLA2G6 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR26992-82,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein Calcium-independent Phospholipase A2 also known as iPLA2 or PLA2G6 is an important enzyme involved in phospholipid metabolism. This enzyme exhibits a catalytic function that does not require calcium for activation which distinguishes it from other members of the phospholipase A2 family. It possesses a molecular weight of approximately 85 kDa. This protein mainly expresses in cytosolic compartments within several tissues including the heart brain and pancreas playing a diverse role in cellular activities.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein catalyzes the hydrolysis of ester bonds within phospholipids releasing free fatty acids and lysophospholipids. This enzyme does not operate as part of a larger complex but functions independently within the cell membrane and metabolic pathways. By regulating phospholipid turnover and remodeling the protein plays a vital role in membrane homeostasis and signaling. This remodeling contributes to cell survival and apoptosis depending on cellular needs and conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis enzyme interacts with lipid signaling and metabolism in central biological processes. It plays a significant role in the glycerophospholipid and arachidonic acid pathways. In these pathways iPLA2 is closely related to cyclooxygenase and lipoxygenase enzymes which convert arachidonic acid into prostaglandins and leukotrienes leading to the mediation of inflammation and other signaling pathways. This interaction emphasizes the enzyme's importance in both normal physiology and pathophysiological conditions.\u003cbr\u003e\nMutations or altered activities of iPLA2 have associations with neurodegenerative disorders such as Parkinson’s disease and infantile neuroaxonal dystrophy. In these disorders mitochondrial dysfunction often linked with proteins like alpha-synuclein becomes prevalent demonstrating the enzyme's influence on apoptosis and oxidative stress. Furthermore iPLA2 mutations may lead to cardiomyopathy where its function overlaps with creatine kinase and enzymes involved in energy metabolism indicating a broader impact on cardiac health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852402151593,"sku":"ab309595","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab309595","provider":"Iright","version":"1.0","type":"link"}