{"product_id":"abcam-ab314153","title":"Abcam, ab314153, Anti-Syntaxin 1B antibody [EPR25899-7] - BSA and Azide free (Detector)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Syntaxin antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR25899-7,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab314153 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Syntaxin 1B. The recommended pair for sandwich ELISA is:\u003cbr\u003e\nCapture:\u003cbr\u003e\nab314152\u003cbr\u003e\n, Syntaxin 1A + Syntaxin 1B Capture Antibody (unconjugated)\u003cbr\u003e\nDetector: ab314153, Syntaxin 1B Detector Antibody (unconjugated)\u003cbr\u003e\nThe reference range value is 0.78-50 ng\/ml.\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nOur carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg\/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSyntaxin 1B often abbreviated as STX1B is an essential SNARE protein with a mass of approximately 33 kDa. This protein is mainly expressed in neurons and tissues with neural characteristics indicating its prominent role in the nervous system. Mechanically Syntaxin 1B facilitates the docking and fusion of synaptic vesicles with the presynaptic membrane. Through this mechanism it aids in the release of neurotransmitters which is fundamental for synaptic transmission and communication between neurons.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein functions as a core component of the SNARE complex comprising Syntaxin 1B SNAP-25 and VAMP2. The formation of this complex brings the synaptic vesicle and presynaptic membrane into close proximity creating the condition for vesicle fusion. Syntaxin 1B plays a significant role in synaptic efficiency and plasticity impacting processes such as learning and memory.\u003cbr\u003e\nPathways\u003cbr\u003e\nSyntaxin 1B is integral to the neurotransmitter release pathway and exocytosis pathways. It closely interacts with proteins such as SNAP-25 and VAMP2 both of which are critical for the proper execution of synaptic vesicle release. Within these pathways Syntaxin 1B assists in the regulated release of chemical messengers ultimately influencing neuronal communication and overall neural network activity.\u003cbr\u003e\nMutations or dysfunctions in Syntaxin 1B have links to neurological conditions such as epilepsy and intellectual disability. Research indicates that abnormalities in Syntaxin 1B might disrupt synaptic transmission potentially through altered interactions with proteins like SNAP-25. These disruptions can lead to erratic neuronal firing patterns or inadequate synaptic connectivity contributing to the pathophysiology observed in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852513038505,"sku":"ab314153","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab314153","provider":"Iright","version":"1.0","type":"link"}