{"product_id":"abcam-ab314534","title":"Abcam, ab314534, Recombinant human ENPP3\/B10 protein - Active (His tag)","description":"\u003cp\u003eSize: 20µg \/ 100µg\u003cbr\u003e\nRecombinant human ENPP3\/B10 protein - Active (His tag) is a Human Fragment protein, in the 48 to 875 aa range, expressed in Mammalian, with \u0026gt;95%, \u0026lt; 1 EU\/µg endotoxin level, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:Mammalian,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Measured by its binding ability in a functional ELISA. AB314534 at 2 μg\/mL can bind an anti-ENPP3 recombinant antibody, the EC50 is 2.151-2.492 ng\/mL.,\u003cbr\u003e\nAccession:O14638,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in water,\u003cbr\u003e\nStorage buffer:pH: 7.4 - 8Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg\/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC\/-80degC. Our default final concentration of glycerol is 50%.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, -80°C, Appropriate long-term storage conditions--20°C, -80°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ENPP3\/B10 protein also known as Ectonucleotide Pyrophosphatase\/Phosphodiesterase 3 is an enzyme that plays a role in nucleotide metabolism by hydrolyzing phosphodiester and phosphosulfate bonds. This protein has a molecular mass of around 100 kDa. ENPP3 is mainly expressed on the surface of epithelial cells and you find it in tissues like the kidney liver and lung. In some contexts immune cells such as mast cells also show its expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nENPP3 regulates the availability of extracellular nucleotides and phosphate metabolites. It is not typically considered part of a larger complex and rather contributes to various processes independently. By modulating concentrations of these extracellular molecules ENPP3 impacts cell signaling and nucleotide homeostasis which can affect cell proliferation and differentiation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis enzyme engages in the purinergic signaling pathway and can influence the MAPK\/ERK pathway. Through its enzymatic action on nucleotides ENPP3 helps regulate signaling molecules which participate in processes such as cell growth and immune responses. Proteins related to these pathways include ATP-binding proteins like P2Y receptors and kinases involved in MAPK\/ERK signaling.\u003cbr\u003e\nENPP3's function links it to conditions such as allergy and asthma as its expression on mast cells affects histamine release. Changes in its activity are also noted in certain cancers where its role in signaling pathways can contribute to uncontrolled cell proliferation. In these disease contexts the protein interacts with other signaling molecules like cytokine receptors and oncogenic proteins which can modulate or exacerbate disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843996274857,"sku":"ab314534","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab314534","provider":"Iright","version":"1.0","type":"link"}