{"product_id":"abcam-ab317271","title":"Abcam, ab317271, Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Multiclonal NDUFB8 antibody. Carrier free. Suitable for IHC-P, ICC\/IF, Flow Cyt (Intra), WB and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Multiclonal,\u003cbr\u003e\nClone number:RM1134,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, ICC\/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody cocktail does cross-react with NDUFB8, SDHB, UQCRC2 and ATP5A from mouse and rat samples.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab317271 is the carrier-free version of\u003cbr\u003e\nab317270\u003cbr\u003e\nWhat are recombinant multiclonals?\u003cbr\u003e\nRecombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:\u003cbr\u003e\n- The sensitivity of polyclonal antibodies by recognising multiple epitopes\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nView our range of\u003cbr\u003e\nrecombinant multiclonal antibodies\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Total OXPHOS Antibody Cocktail targets key proteins involved in oxidative phosphorylation (OXPHOS) a critical process for ATP production in mitochondria. It includes antibodies against five complexes of the electron transport chain often known as complexes I-V. These complexes are expressed in mitochondria within almost all eukaryotic cells especially high-energy demanding tissues like muscle and brain. The molecular weight of these complex components varies but each plays an important role in transferring electrons during ATP synthesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThese complexes form the core of the electron transport chain an assembly of protein complexes embedded in the mitochondrial inner membrane. OXPHOS as part of this chain is important for cellular respiration and metabolic energy production. Proteins within these complexes work together to generate a proton gradient that drives ATP synthase activity. The efficient function of this system supports high demands of cellular energy and is essential for maintaining metabolic homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe Total OXPHOS Antibody Cocktail components integrate into the oxidative phosphorylation and citric acid cycle pathways. Energy transduction from NADH and FADH2 through the electron transport chain connects OXPHOS with these pathways. Cytochrome c an important protein acts as an electron shuttle between complex III and complex IV within this process highlighting its importance for proper function and energy production.\u003cbr\u003e\nIrregularities in OXPHOS function link to disorders such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These conditions often result from mutations impacting the electron transport chain proteins disrupting ATP production and leading to energy deficits in cells. For instance mutations in NADH:ubiquinone oxidoreductase (complex I) contribute to disease development emphasizing the need for understanding these targets to develop potential therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850189131945,"sku":"ab317271","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab317271","provider":"Iright","version":"1.0","type":"link"}