{"product_id":"abcam-ab317548","title":"Abcam, ab317548, Influenza A Virus Hemagglutinin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nInfluenza A Virus Hemagglutinin ELISA Kit is a single-wash 90-min Simplestep used to quantify Influenza A Virus Hemagglutinin with a sensitivity of 18.6 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Urine, Saliva,\u003cbr\u003e\nReacts with:Influenza A,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 18.6 pg\/mL,\u003cbr\u003e\nRange:101.563 - 6500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nInfluenza A Virus Hemagglutinin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemagglutinin protein in Cell culture media. Quantitate Influenza A Virus Hemagglutinin with 18.6 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\ntechnology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details. Our SimpleStep ELISA\u003cbr\u003e\ntechnology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)\u003cbr\u003e\n-Also available in a fully validated 384-well format.\u003cbr\u003e\nThis kit was made against Influenza A H1N1\/Peurto Rico\/8\/1934. Cross reactivity was tested for Influenza A H3N2 (A\/Darwin\/9\/2021), Influenza A H5N1 (A\/Egypt\/N000\/2015), and Influenza A H1N1 (A\/California\/07\/2009). No cross reactivity was observed.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInfluenza A Virus Hemagglutinin also known as hemagglutinin or influenza HA is a surface glycoprotein found on the influenza A virus. Hemagglutinin has a mass of approximately 75 kDa and serves as an important player in the virus's ability to infect host cells. It is expressed on the exterior membrane of the influenza virus and allows the virus to attach to and enter host cells initiating the infection process. This protein comes in various subtypes adding complexity to the virus's infection strategies and complicating vaccine development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHemagglutinin facilitates the binding of the virus to sialic acid receptors on the surface of host epithelial cells. This binding triggers endocytosis allowing the virus entry into the host cell. Hemagglutinin is part of a complex that works closely with neuraminidase another glycoprotein on the virus surface. The hemagglutinin-neuraminidase complex is essential for the viral life cycle balancing attachment to host cells with the release of new viral particles.\u003cbr\u003e\nPathways\u003cbr\u003e\nHemagglutinin plays a significant role in the viral infection pathway of the influenza virus. It is critical in the initial steps of the pathway that involve viral entry and fusion with the host cell membrane. Hemagglutinin's interaction with the sialic acid receptors is closely linked with the activity of neuraminidase which aids in the release stage of the infection. This interplay between hemagglutinin and neuraminidase highlights their participation in the viral replication cycle demonstrating their complementary roles.\u003cbr\u003e\nHemagglutinin is deeply connected to seasonal influenza outbreaks and pandemics. Alterations in the hemagglutinin protein such as mutations and antigenic drifts or shifts can lead to new influenza strains with pandemic potential. The interaction of hemagglutinin with host immune responses and its capability to change allows the virus to evade immune detection. Neuraminidase inhibitors a class of antiviral drugs target enzymes closely related to hemagglutinin to prevent the spread of infection showcasing hemagglutinin's centrality in both infection and therapeutic intervention strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843630289065,"sku":"ab317548","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab317548","provider":"Iright","version":"1.0","type":"link"}