{"product_id":"abcam-ab321949","title":"Abcam, ab321949, BOND RX - Anti-Smad3 (phospho S423 + S425) antibody [EP823Y]","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRabbit Recombinant Monoclonal SMAD3 (pS423\/425) + SMAD5 (pS463\/465 ) + SMAD1 (pS463\/465) + SMAD2 (pS465\/467) antibody. Suitable for detection of SMAD3 + SMAD5 + SMAD2 in IHC. Suitable for human and mouse samples. This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-Smad3 (phospho S423 + S425) antibody [EP823Y] - ab52903. - Validated on the Leica BOND RX automated IHC staining platform for SMAD3 + SMAD5 + SMAD2 IHC - Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation - EP823Y clone cited in over 570 publications - Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency\u003cbr\u003e\nProduct details:\u003cbr\u003e\nThis product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-Smad3 (phospho S423 + S425) antibody [EP823Y] -\u003cbr\u003e\nab52903\u003cbr\u003e\nAdditional Titration Inserts can be purchased from Leica Biosystems, using OPT9719 (box of 50). Or Titration Container\/Insert Kit, using OPT9049 (box of 10\/50).\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSmad3 also known as Mothers against decapentaplegic homolog 3 is a protein that plays a mechanical role in signal transduction. It acts mainly as a transcription factor and gets activated through phosphorylation. The molecular weight of Smad3 is approximately 48 kDa. It is expressed widely across numerous tissues including the cellular nucleus where it executes its function after activation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSmad3 acts as a mediator of signal transduction for the TGF-beta (transforming growth factor-beta) superfamily forming a complex with phosphorylated Smad2. This enables it to regulate transcriptional activity influencing cell proliferation differentiation and apoptosis. Smad3 also participates in various cellular processes by interacting with other co-factors and regulatory proteins that aid in fine-tuning its function.\u003cbr\u003e\nPathways\u003cbr\u003e\nSmad3 plays an important role in the TGF-beta signaling pathway where it works closely with Smad4 to propagate the signal. Upon phosphorylation it forms a complex with co-Smad (Smad4) and moves into the nucleus to influence gene expression. Smad3 is also involved in pathways related to oncogenesis and tissue fibrosis indicating its significant role in cellular regulation and response mechanisms.\u003cbr\u003e\nSmad3 is associated with fibrotic diseases and cancers particularly in tissues such as the liver and lungs. Altered Smad3 signaling contributes to the pathological process occurring in fibrotic disorders often interacting with Smad4 in these abnormalities. Dysregulated Smad3 expression or mutations can also lead to oncogenic transformations highlighting its critical involvement in disease states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843615445161,"sku":"ab321949","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab321949","provider":"Iright","version":"1.0","type":"link"}