{"product_id":"abcam-ab325292","title":"Abcam, ab325292, Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568) - FluoTag®-X2","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nFully defined \u0026amp; ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates featuring FluoTag® and Smart Secondaries® technologies.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Alpaca,\u003cbr\u003e\nTarget species:Rabbit,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Fc region,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nConjugation:AZdye 568,\u003cbr\u003e\nExcitation\/Emission:Ex: 578nm, Em: 602nm,\u003cbr\u003e\nApplications:ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:10E10,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nFluoTags®\u003cbr\u003e\nFluoTags are fully defined \u0026amp; ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.\u003cbr\u003e\nTogether with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.\u003cbr\u003e\nFluoTag®-X2 (One target - One sdAb – Two fluorophores (1:1:2)\u003cbr\u003e\nFluoTag®-X2 reagents are composed of a single sdAb carrying two fluorophores per protein molecule. The maximal displacement of fluorophores from the antigen-binding surface is ~3 nm. FluoTag®-X2 reagents show good tissue penetration features.\u003cbr\u003e\nFeatures:\u003cbr\u003e\nHigh affinity monovalent binding + two fluorophores per sdAb for brighter signals\u003cbr\u003e\nReagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.\u003cbr\u003e\nSmart Secondaries®\u003cbr\u003e\nSmart Secondaries® are a unique set of high-affinity single-domain antibodies recognizing conventional immunoglobulins with exquisite species and isotype specificity. They provide unique benefits for all antibody-based assays in research and diagnostics by combining reproducible specificity and production with small and high-affinity monovalent binding. The technology offers surprising new possibilities like one-step indirect immunofluorescence or easy multiplexing.\u003cbr\u003e\nIn comparison to conventional secondary reagents, Smart Secondary® nanobodies feature significant advantages:\u003cbr\u003e\nSmall probes for enhanced staining speed and improved tissue penetration and higher resolution.\u003cbr\u003e\nMonoclonal tools: Clearly defined species specificity – once and forever!\u003cbr\u003e\nIsotype specificity for independent detection of mouse IgG1 and IgG2a\/b isotypes.\u003cbr\u003e\nRecombinant production for reliable quality, scalability and reproducibility without using animals.\u003cbr\u003e\nMonovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible!\u003cbr\u003e\nExtraordinarily strong binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing)\u003cbr\u003e\nEquipped with propriety FX technology for improved post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Size-exclusion chromatography, Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1 month, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847570247849,"sku":"ab325292","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab325292","provider":"Iright","version":"1.0","type":"link"}