{"product_id":"abcam-ab3778","title":"Abcam, ab3778, Anti-Aggrecan antibody [6-B-4]","description":"\u003cp\u003eSize: 20µg \/ 100µg \/ 1mg\u003cbr\u003e\nAnti-Aggrecan antibody [6-B-4] (ab3778) is a mouse monoclonal antibody detecting Aggrecan in  Western Blot, IHC-P . Suitable for  Human . - Over 190 publications - Trusted since 2003\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:6-B-4,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with this antibody. However, there are many citations indicating this antibody is able to detect Aggrecan in endogenous sample lysates.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Aggrecan antibody [6-B-4] (ab3778) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, samples.\u003cbr\u003e\nWhat is the molecular weight of Aggrecan?\u003cbr\u003e\nAnti-Aggrecan [6-B-4] (ab3778) specifically detects a band for Aggrecan (UniProt: P16112) at a molecular weight of 250kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Aggrecan [6-B-4] (ab3778) was first used in a scientific publication in 2003 and has been cited over 190 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-Aggrecan [6-B-4] (ab3778) has over 5 independent reviews from customers.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [6-B-4] also available for your convenience: ab3778, Carrier free -\u003cbr\u003e\nab255969\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab315140\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab318312\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAggrecan also known as aggrecan core protein or chondroitin sulfate proteoglycan 1 is a large proteoglycan vital for cartilage structure and function. It has a mass of approximately 250 kDa. Aggrecan is expressed mostly in cartilage regions such as articular cartilage but it might also be found in other connective tissues. The structure of aggrecan consists of a core protein that attaches to glycosaminoglycan chains allowing it to create hydrated gel structures essential for the resilience and load-bearing properties of cartilage.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAggrecan plays a significant role in maintaining the structural integrity of cartilage by providing resistance to compression. This proteoglycan is a central component of the extracellular matrix forming large aggregates with hyaluronic acid and link proteins. These aggregates contribute to the cartilage's osmotic properties helping resist compression during joint movement. Aggrecan's structural properties make it necessary for normal joint function and health.\u003cbr\u003e\nPathways\u003cbr\u003e\nAggrecan interacts within the cartilage development and maintenance frameworks. Specifically it participates in the regulation of the ECM-receptor interaction pathway and the proteoglycans in cancer pathway. Aggrecan functions alongside proteins such as type II collagen to ensure biomechanical properties of the cartilage. Additionally its enzymatic breakdown by aggrecanases like ADAMTS can impact these pathways leading to various physiological effects.\u003cbr\u003e\nAggrecan is closely associated with osteoarthritis and intervertebral disc degeneration. In osteoarthritis the loss of aggrecan through enzymatic degradation leads to reduced joint function and increased pain. The breakdown involves aggrecanases and other matrix metalloproteinases resulting in cartilage degradation. Diseases such as intervertebral disc degeneration also link to the reduced presence or altered structure of aggrecan impacting spine health and mobility.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844075409577,"sku":"ab3778","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab3778","provider":"Iright","version":"1.0","type":"link"}