{"product_id":"abcam-ab40759","title":"Abcam, ab40759, Anti-Smad4 antibody [EP618Y]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-Smad4 antibody [EP618Y] (ab40759) is a rabbit monoclonal antibody detecting Smad4 in  Western Blot, IHC-P . Suitable for  Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 170 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP618Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-Smad4 antibody [EP618Y] (ab40759) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in human, mouse, rat samples.\u003cbr\u003e\nAnti-Smad4 antibody [EP618Y] (ab40759) specifically detects Smad4 (UniProt ID: Q13485; Molecular weight: 60kDa) and is sold in 100 µL and 1 mL selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-Smad4 antibody [EP618Y] (ab40759) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-Smad4 antibody [EP618Y] (ab40759) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-Smad4 antibody [EP618Y] (ab40759) has been cited over 170 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-Smad4 antibody [EP618Y] (ab40759) has 9 independent reviews from customers.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nAntibody clone EP618Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (\u003cbr\u003e\nab195554\u003cbr\u003e\nab311050\u003cbr\u003e\nab311176\u003cbr\u003e\nab311831\u003cbr\u003e\nab313114\u003cbr\u003e\nab313307\u003cbr\u003e\nab321616\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSmad4 also known as DPC4 or MADH4 is a central protein in the TGF-beta signaling pathway with a molecular mass of approximately 60 kDa. It acts as a signal transducer that facilitates communication from the cell surface to the nucleus. Smad4 is broadly expressed in various tissues playing an important role in the regulation of cellular processes. It forms a complex with receptor-regulated Smads (R-Smads) to translocate to the nucleus where it influences gene transcription.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSmad4 influences cell proliferation differentiation and apoptosis by mediating signals from TGF-beta cytokines. It is part of the Smad protein family acting as a transcriptional controller. Upon TGF-beta receptor activation Smad4 forms complexes with Smad2 and Smad3 translocating to the nucleus to regulate genes imperative for cellular homeostasis. Its role in cell cycle regulation underlines its contribution to normal cellular functions and its potential involvement in disorders.\u003cbr\u003e\nPathways\u003cbr\u003e\nSmad4 operates within the TGF-beta pathway linking extracellular signals to nuclear transcription alterations. It participates in the regulation of epithelial-mesenchymal transition (EMT) a process important for development and tumor progression. In these pathways Smad4 interacts closely with Smad2 and Smad3 orchestrating various cellular responses to external stimuli through transcriptional management.\u003cbr\u003e\nSmad4 is highly related to cancer and juvenile polyposis syndrome. Mutations or deletions in Smad4 disrupt its function contributing to the progression of pancreatic cancer and colorectal cancer among others. Within these contexts Smad4 connects strongly to other proteins like p21 and cyclin-dependent kinase inhibitors which are important in cell cycle arrest and impede tumor growth.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844046606505,"sku":"ab40759","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab40759","provider":"Iright","version":"1.0","type":"link"}