{"product_id":"abcam-ab4539","title":"Abcam, ab4539, Anti-CEACAM 5 + 6 antibody [MUS]","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nMouse Monoclonal CEACAM5 antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB, IHC-Fr and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CEACAM5.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:MUS,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:unknown,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-Fr, WB, ELISA, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Native Full Length Protein corresponding to Human CEACAM5.P06731,\u003cbr\u003e\nSpecificity:MUS reacts specifically with CEACAM 5 (CEA\/CD66e) and CEACAM 6 (NCA\/CD66c) transiently expressed on the cell surface of transfected BOSC23 cells as demonstrated by flow cytometry.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAntibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCEACAM5 and CEACAM6 also known as carcinoembryonic antigen-related cell adhesion molecule 5 and 6 are glycoproteins with important mechanical roles in cellular adhesion. CEACAM5 has a molecular weight of approximately 180 kDa and CEACAM6 weighs around 90 kDa. These proteins mainly express in epithelial cells especially in mucosal tissues such as the gastrointestinal tract and respiratory epithelium. CEACAM5 was also identified as the carcinoembryonic antigen (CEA) widely utilized in cancer diagnostics.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe CEACAM proteins including CEACAM5 and CEACAM6 contribute to cellular adhesion processes and signal transduction. They function as part of larger complexes that mediate cell-cell interactions. By engaging in homophilic and heterophilic binding they help regulate cell proliferation and differentiation. In normal physiological conditions these proteins play roles in maintaining tissue architecture and integrity.\u003cbr\u003e\nPathways\u003cbr\u003e\nCEACAM5 and CEACAM6 proteins involve cellular communication and immune system pathways. They interact with signaling molecules part of pathways like the MAPK and PI3K-Akt pathways influencing cellular responses to external stimuli. Related proteins such as CEACAM1 can modulate immune reactions and cell signaling showing the interconnected roles of CEACAM family members in these pathways.\u003cbr\u003e\nCEACAM5 and CEACAM6 connect strongly with various cancers particularly colorectal and lung cancer. Overexpression of these proteins often indicates tumor progression and metastasis. CEACAM5 is frequently used as a marker for colorectal cancer prognosis while CEACAM6 is associated with the invasive nature of cancer cells. Both proteins interact with pathways involving epidermal growth factor receptor (EGFR) highlighting their relevance in oncogenic processes and potential as therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854638993577,"sku":"ab4539","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab4539","provider":"Iright","version":"1.0","type":"link"}