{"product_id":"abcam-ab46027","title":"Abcam, ab46027, Human IL-6 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 2 x 96Tests\u003cbr\u003e\nHuman IL-6 ELISA Kit is a sandwich ELISA designed to quantify Human IL-6 with a sensitivity of 2 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 6.25 - 200 pg\/mL - Cited in over 80 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 2 pg\/mL,\u003cbr\u003e\nRange:6.25 - 200 pg\/mL,\u003cbr\u003e\nAssay time:1h 45m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-6 ELISA Kit ab46027 is a sandwich ELISA to measure Human IL-6 in serum, plasma, cell culture supernatant with a sensitivity of 2 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman IL-6 ELISA Kit ab46027 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used\u003cbr\u003e\n2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature\u003cbr\u003e\n3. Aspirate and wash each well\u003cbr\u003e\n4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature\u003cbr\u003e\n5. Aspirate and wash each well.\u003cbr\u003e\n6. Add the TMB Solution to each well until color develops and then add the Stop Solution\u003cbr\u003e\n7. Immediately begin recording the color development\u003cbr\u003e\nGet results in 90 minutes with our SimpleStep ELISA\u003cbr\u003e\nHuman IL-6 ELISA Kit (\u003cbr\u003e\nab178013\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInterleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein's function and presence in experimental and clinical settings.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.\u003cbr\u003e\nPathways\u003cbr\u003e\nIL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.\u003cbr\u003e\nIL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843595554985,"sku":"ab46027","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab46027","provider":"Iright","version":"1.0","type":"link"}