{"product_id":"abcam-ab46035","title":"Abcam, ab46035, Human IL-12 ELISA Kit High Sensitivity","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman IL-12 ELISA Kit High Sensitivity is a sandwich ELISA designed to quantify Human IL-12 with a sensitivity of 0.75 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.78 - 25 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 0.75 pg\/mL,\u003cbr\u003e\nRange:0.78 - 25 pg\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-12 ELISA Kit High Sensitivity ab46035 is a sandwich ELISA to measure Human IL-12 High Sensitivity in serum, plasma, cell culture supernatant with a sensitivity of 0.75 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IL-12 ELISA kit High Sensitivity is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and cell culture medium.\u003cbr\u003e\nA monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples.\u003cbr\u003e\nThis kit will recognize both endogenous and recombinant Human IL-12.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman IL-12 ELISA Kit High Sensitivity ab46035 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used.\u003cbr\u003e\n2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature.\u003cbr\u003e\n3.Aspirate and wash each well.\u003cbr\u003e\n4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature.\u003cbr\u003e\n5. Aspirate and wash each well.\u003cbr\u003e\n6. Add the TMB Solution to each well until color develops and then add the Stop Solution.\u003cbr\u003e\n7. Immediately begin recording the color development\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843624784041,"sku":"ab46035","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab46035","provider":"Iright","version":"1.0","type":"link"}