{"product_id":"abcam-ab5439","title":"Abcam, ab5439, Anti-Hsp70 antibody [3A3]","description":"\u003cp\u003eSize: 20µg \/ 100µg \/ 1mg\u003cbr\u003e\nAnti-Hsp70 antibody [3A3] (ab5439) is a mouse monoclonal antibody detecting Hsp70 in  Western Blot, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - Over 60 publications - Trusted since 2003\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:3A3,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IHC-P, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nEpitope:Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human Hsp 70, a region that has been shown to be involved in stress-induced nucleolar localization.,\u003cbr\u003e\nSpecificity:This antibody detects several members of the heat shock protein 70 kDa (Hsp 70) gene family including Hsp 70, Hsc 70, p75, and following heat shock, Hsp 72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Hsp70 antibody [3A3] (ab5439) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Hsp70?\u003cbr\u003e\nAnti-Hsp70 [3A3] (ab5439) specifically detects a band for Hsp70 (UniProt: P0DMV9) at a molecular weight of 70-78kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Hsp70 [3A3] (ab5439) was first used in a scientific publication in 2003 and has been cited over 60 times in peer-reviewed journals.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [3A3] also available for your convenience: ab5439, Carrier free -\u003cbr\u003e\nab252240\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab309609\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHsp70 also known as Heat Shock Protein 70 or HSPA1B is a molecular chaperone with a mass of approximately 70 kDa. It plays a mechanical role by assisting in the proper folding of nascent polypeptide chains and the refolding of misfolded proteins. Researchers often detect Hsp70 using Western blot and immunohistochemistry (IHC) techniques. Hsp70 is widely expressed in many tissues particularly during stress conditions like heat shock where its expression level increases significantly.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHsp70 operates by stabilizing intermediate states of folding proteins preventing aggregation and facilitating the correct folding process. It often forms a complex with co-chaperones such as Hsp40 and nucleotide exchange factors. This complex is essential for the protein's activity and function. Additionally Hsp70 participates in protein degradation pathways by guiding misfolded proteins to the proteasome for degradation maintaining cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis molecular chaperone plays significant roles in the heat shock response and unfolded protein response pathways. Hsp70 interacts closely with proteins such as Hsp90 and co-chaperones which together help protect cells from stress-induced damage. The protein also participates in the JAK\/STAT signaling pathway influencing cell proliferation and apoptosis. These interactions suggest an integral role in maintaining cellular integrity during stress conditions.\u003cbr\u003e\nOverexpression of Hsp70 has been associated with various cancers and neurodegenerative diseases. In cancer Hsp70 helps tumor cells survive the hostile tumor microenvironment partly by interacting with anti-apoptotic proteins such as Bcl-2. In neurodegenerative disorders such as Alzheimer's disease Hsp70 associates with amyloid-beta peptides potentially mitigating their aggregation toxicity. These interactions highlight Hsp70's importance in both protective and pathological cellular processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844055650473,"sku":"ab5439","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab5439","provider":"Iright","version":"1.0","type":"link"}