{"product_id":"abcam-ab59203","title":"Abcam, ab59203, Anti-Myosin Phosphatase (phospho T853) antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRabbit Polyclonal Myosin Phosphatase phospho T853 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R12A phospho T853.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human PPP1R12A phospho T853. The exact immunogen used to generate this antibody is proprietary information.O14974,\u003cbr\u003e\nSpecificity:Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat)\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMyosin phosphatase sometimes also known as Myosin Light Chain Phosphatase is an enzyme that plays a mechanical role in the dephosphorylation of myosin light chains which are important for muscle contraction and cell motility. This enzyme typically consists of three subunits: a catalytic subunit known as protein phosphatase 1 and two regulatory subunits. Together they generally create a structure with a mass between 100-150 kDa. You will find myosin phosphatase widely expressed across muscle tissues such as cardiac smooth and skeletal muscles.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMyosin phosphatase regulates contraction by reversing phosphorylation of myosin light chains an important process in muscle function and various cellular activities. This enzyme operates as part of the myosin phosphatase complex where it provides the capability needed to maintain equilibrium between phosphorylating and dephosphorylating processes. By controlling this balance myosin phosphatase plays a significant role in cell movement cell shape and tension maintenance.\u003cbr\u003e\nPathways\u003cbr\u003e\nMyosin phosphatase is integrated into the RhoA\/Rho-kinase signaling pathway which is important for cytoskeletal dynamics and cell adhesion. Within this pathway myosin phosphatase interacts with proteins like Rho-kinase (ROCK) which counter-regulates its activity by inhibiting dephosphorylation. The crosstalk with another protein protein phosphatase 1 is also significant in controlling phosphorylation states and facilitating cellular responses to external stimuli.\u003cbr\u003e\nMyosin phosphatase is involved in hypertension and heart failure through its action on smooth muscle contraction. Abnormal regulation and activity of this enzyme can lead to unwanted contraction and subsequently increased blood pressure. The protein Rho-kinase is implicated in these conditions as it often modifies the activity of myosin phosphatase. Furthermore deregulation of the associated cellular pathways contributes to cardiac stress and dysfunction highlighting its involvement in heart-related pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848619643049,"sku":"ab59203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab59203","provider":"Iright","version":"1.0","type":"link"}